Accueil>>Signaling Pathways>> Endocrinology and Hormones>> FXR & LXR>>24α-methyl Cholesterol

24α-methyl Cholesterol (Synonyms: Campesterol,NSC 224330)

Catalog No.GC13431

24α-methyl Cholestérol est un stérol végétal ayant des effets hypocholestérolémiants et anticancérigènes.

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24α-methyl Cholesterol Chemical Structure

Cas No.: 474-62-4

Taille Prix Stock Qté
1mg
40,00 $US
En stock
5mg
134,00 $US
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment:

The effect of campesterol on the viable cell number was assessed by XTT assay. HUVECs were treated with various concentrations (10, 20, 30, 40 and 50 μg/mL) of campesterol and incubated at 37 °C in a humidified incubator for 24 h, a XTT working solution was added to each well. The cells were incubated at 37 °C for 2 h and the optical density was measured using a microplate reader at 450 nm[1].

References:

[1]. Choi JM, et al. Identification of campesterol from Chrysanthemum coronarium L. and its antiangiogenic activities. Phytother Res. 2007 Oct;21(10):954-9.

Background

24α-methyl Cholesterol is an agonist of liver X receptors (LXR).

Liver X receptors (LXRs) are ligand-dependent transcription factors involved in the transcriptional control of lipid metabolism. LXRs function as nuclear cholesterol sensors that are activated by elevated intracellular cholesterol levels in multiple cell types. Activation of LXRs induces the expression of various genes involved in cholesterol absorption, transport, efflux, and excretion. In addition to their function in lipid metabolism, LXRs have also been found to modulate immune and inflammatory responses in macrophages. LXRα is highly expressed in the liver and at lower levels in the adrenal glands, adipose, intestine, lung, macrophages, and kidney, and LXRβ is ubiquitously expressed [2].

24α-methyl Cholesterol was a phytosterol found in vegetables, nuts, fruits, and seeds that competitively inhibited the absorption of intestinal cholesterol and decreased the transcription of genes important for cholesterol metabolism [3]. 24α-methyl Cholesterol acted as an agonist at liver X receptors (LXR) and suppressed the proliferation of prostate and breast cancer cells [1].

References:
[1] Chuu C P, Kokontis J M, Hiipakka R A, et al.  Modulation of liver X receptor signaling as novel therapy for prostate cancer[J]. Journal of biomedical science, 2007, 14(5): 543-553.
[2] Zelcer N, Tontonoz P.  Liver X receptors as integrators of metabolic and inflammatory signaling[J]. The Journal of clinical investigation, 2006, 116(3): 607-614.
[3] Segura R, Javierre C, Lizarraga M A, et al.  Other relevant components of nuts: phytosterols, folate and minerals[J]. British Journal of Nutrition, 2006, 96(S2): S36-S44.

Chemical Properties

Cas No. 474-62-4 SDF
Synonymes Campesterol,NSC 224330
Chemical Name (3β,24R)-ergost-5-en-3-ol
Canonical SMILES O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CC[C@@H](C)C(C)C
Formula C28H48O M.Wt 400.7
Solubility ≤0.25mg/ml in ethanol; 1mg/ml in dimethyl formamide; 0.8mg/ml in Water (ultrasonic and warming and heat) Storage Store at 2-8°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.4956 mL 12.4782 mL 24.9563 mL
5 mM 0.4991 mL 2.4956 mL 4.9913 mL
10 mM 0.2496 mL 1.2478 mL 2.4956 mL
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