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3x DYKDDDDK Tag

Catalog No.GC72182

3x DYKDDDDK Tag est un outil utile pour étudier la fonction et la localisation de protéines dont les anticorps (Abs) ne sont pas disponibles.

Products are for research use only. Not for human use. We do not sell to patients.

3x DYKDDDDK Tag Chemical Structure

Cas No.: 921765-76-6

Taille Prix Stock Qté
1 mg
61,00 $US
En stock
5 mg
178,00 $US
En stock
10 mg
292,00 $US
En stock

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Sample solution is provided at 25 µL, 10mM.

Description Chemical Properties Product Documents
3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance .

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].

References:
[1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830.
[2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.

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