4-O10b1 |
| Catalog No.GC92085 |
4-O10b1 est un lipide cationique ionisable (pKa = 5,148) qui a été utilisé dans la génération de nanoparticules lipidiques (LNP) pour la délivrance de siRNA in vitro et in vivo.
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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4-O10b1 is an ionizable cationic lipid (pKa = 5.148) that has been used in the generation of lipid nanoparticles (LNPs) for the delivery of siRNA in vitro and in vivo.1 LNPs containing 4-O10b1, conjugated to an antibody targeting the mouse macrophage marker F4/80 (anti-F4/80), and encapsulating siRNA targeting the mRNA encoding TGF-β-activated kinase 1 (Tak1) inhibit poly(I:C)-induced production of IL-1β, IL-6, and TNF-α in RAW 264.7 macrophages. In vivo, intranasal administration of these LNPs decreases lung injury and reduces total protein levels and myeloperoxidase (Mpo) activity in bronchoalveolar lavage fluid (BALF) in a mouse model of influenza A-induced pneumonia.
References:
[1]. Zhao, G., Xue, L., Geisler, H.C., et al.Precision treatment of viral pneumonia through macrophage-targeted lipid nanoparticle deliveryProc. Natl. Acad. Sci. USA121(7)e2314747121(2024).
| Cas No. | SDF | ||
| Chemical Name | tetra(decan-2-yl) 3,3',3'',3'''-((piperazine-1,4-diylbis(propane-3,1-diyl))bis(azanetriyl))tetrapropionate | ||
| Canonical SMILES | CC(CCCCCCCC)OC(CCN(CCC(OC(C)CCCCCCCC)=O)CCCN1CCN(CCCN(CCC(OC(C)CCCCCCCC)=O)CCC(OC(C)CCCCCCCC)=O)CC1)=O | ||
| Formula | C62H120N4O8 | M.Wt | 1049.7 |
| Solubility | Ethanol: Soluble: ≥10 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.9527 mL | 4.7633 mL | 9.5265 mL |
| 5 mM | 0.1905 mL | 0.9527 mL | 1.9053 mL |
| 10 mM | 0.0953 mL | 0.4763 mL | 0.9527 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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