8-Bromo-cAMP, sodium salt (Synonyms: 8-Br-cAMP, 8-Bromoadenosine 3',5'-cyclic monophosphate, 8-bromo-cAMP) |
| Catalog No.GC16929 |
8-Bromo-cAMP (8-Bromo-cAMP, sodium salt) est un analogue de l'AMPc perméable aux cellules qui agit comme un activateur de la protéine kinase dépendante du CAMP (activateur de la PKA).
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Cas No.: 76939-46-3
Sample solution is provided at 25 µL, 10mM.
8-Bromo-cAMP (8-Bromo-cAMP, sodium salt) est un analogue de l'AMPc perméable aux cellules qui agit comme un activateur de la protéine kinase dépendante du CAMP (activateur de la PKA). Il a la capacité d'inhiber la croissance des cellules tumorales et de faciliter la différenciation des tumeurs [1-2].
8-Bromo-cAMP (20μM;24 h/48h) a induit la différenciation et l'apoptose des cellules de carcinome œsophagien humain Eca-109[3]. 8-Bromo-cAMP (0.1/0.5 mM;10jours) augmente l'efficacité de l'induction de la pluripotence dans les cellules de fibroblastes humains[4].8-Bromo-cAMP (250 μM) inhibe l'activité de transport du glucose dans les cellules placentaires de souris en culture[5]. 8-Bromo-cAMP(0-1mM) induit une réponse proliférative dans une lignée de cellules leucémiques dépendantes de l'IL-3[6].
8-Bromo-cAMP (60 mg/kg/jour ; 7 jours ; i.p.) a significativement inhibé la croissance de la tumeur CT26 chez la souris[7]. 8-Bromo-cAMP renforce les réponses immunitaires à médiation humorale et cellulaire induites par un vaccin ADN contre le VIH-1 chez la souris[8].
References:
[1]. Chen TC, Hinton DR, et,al. Up-regulation of the cAMP/PKA pathway inhibits proliferation, induces differentiation, and leads to apoptosis in malignant gliomas. Lab Invest. 1998 Feb;78(2):165-74. PMID: 9484714.
[2]. Li H, Chen HC, et,al.Identification of a rapidly dephosphorylating 95-kDa protein as elongation factor 2 during 8-Br-cAMP treatment of N1E115 neuroblastoma cells. Biochem Biophys Res Commun. 1995 Dec 5;217(1):131-7. doi: 10.1006/bbrc.1995.2754. PMID: 8526900.
[3]. Wang HM, Zheng NG, et,al. Dual effects of 8-Br-cAMP on differentiation and apoptosis of human esophageal cancer cell line Eca-109. World J Gastroenterol. 2005 Nov 7;11(41):6538-42. doi: 10.3748/wjg.v11.i41.6538. PMID: 16425431; PMCID: PMC4355801.
[4]. Wang Y, Adjaye J. A cyclic AMP analog, 8-Br-cAMP, enhances the induction of pluripotency in human fibroblast cells. Stem Cell Rev Rep. 2011 Jun;7(2):331-41. doi: 10.1007/s12015-010-9209-3. PMID: 21120637.
[5]. Sakata M, Yamaguchi M, et,al. 8-Bromo-cAMP inhibits glucose transport activity in mouse placental cells in culture. J Endocrinol. 1996 Aug;150(2):319-27. doi: 10.1677/joe.0.1500319. PMID: 8869598.
[6]. Barge RM, Falkenburg JH, et,al. 8-Bromo-cAMP induces a proliferative response in an IL-3 dependent leukemic cell line and activates Erk 1,2 via a Shc-independent pathway. Biochim Biophys Acta. 1997 Feb 4;1355(2):141-6. doi: 10.1016/s0167-4889(96)00130-9. PMID: 9042334.
[7]. Wang S, Zhang Z, et,al. Angiogenesis and vasculogenic mimicry are inhibited by 8-Br-cAMP through activation of the cAMP/PKA pathway in colorectal cancer. Onco Targets Ther. 2018 Jul 2;11:3765-3774. doi: 10.2147/OTT.S164982. PMID: 29997437; PMCID: PMC6033084.
[8]. Arai H, Xin KQ, et,al. 8 Br-cAMP enhances both humoral and cell-mediated immune responses induced by an HIV-1 DNA vaccine. Gene Ther. 2000 Apr;7(8):694-702. doi: 10.1038/sj.gt.3301145. PMID: 10800093.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules Eca-109 (cellules de carcinome œsophagien humain) |
Méthode de préparation | Les cellules ont été cultivées avec du 8-Bromo-cAMP pendant 24 heures et 48 heures. |
Conditions de réaction | 20μmol/L;24 h/48h |
Domaines d'application | Le traitement au 8-Bromo-cAMP induit la différenciation et l'apoptose des cellules Eca-109. |
| Expériences animales [2]: | |
Modèles animaux | Souris BALB/c |
Méthode de préparation | Les souris ont subi une implantation de tissu de carcinome CT26 dans leur cæcum. Le groupe expérimental a reçu des injections intrapéritonéales de 8-Bromo-cAMP, tandis que le groupe témoin a reçu une solution saline normale. |
Forme de dosage | 60 mg/kg/jour ; 7 jours ; i.p. |
Domaines d'application | 8-Bromo-cAMP a significativement inhibé la croissance de la tumeur CT26 chez la souris.8-Bromo-cAMP a significativement inhibé la croissance de la tumeur CT26 chez la souris. |
Références : | |
| Cas No. | 76939-46-3 | SDF | |
| Synonymes | 8-Br-cAMP, 8-Bromoadenosine 3',5'-cyclic monophosphate, 8-bromo-cAMP | ||
| Chemical Name | sodium (4aR,6R,7R,7aS)-6-(6-amino-8-bromo-9H-purin-9-yl)-7-hydroxytetrahydro-4H-furo[3,2-d][1,3,2]dioxaphosphinin-2-olate 2-oxide | ||
| Canonical SMILES | NC1=C(N=C(Br)N2[C@@H]3O[C@H](COP(O4)([O-])=O)[C@@H]4[C@H]3O)C2=NC=N1.[Na+] | ||
| Formula | C10H10BrN5NaO6P | M.Wt | 430.09 |
| Solubility | ≥ 43mg/mL in Water;DMSO : 250 mg/mL (581.29 mM; Need ultrasonic) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.3251 mL | 11.6255 mL | 23.2509 mL |
| 5 mM | 0.465 mL | 2.3251 mL | 4.6502 mL |
| 10 mM | 0.2325 mL | 1.1625 mL | 2.3251 mL |
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Quality Control & SDS
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- Purity: >99.50%
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Average Rating: 5 (Based on Reviews and 17 reference(s) in Google Scholar.)
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