A23187 (Synonyms: Calcimycin) |
Catalog No.GC11200 |
A23187 (A-23187) est un antibiotique et un ionophore de cations divalents unique (comme le calcium et le magnésium).
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 52665-69-7
Sample solution is provided at 25 µL, 10mM.
A23187, free acid is a Ca2+ ionophore [1].
Ca2+ ionophore acts as a Ca2+ ionophore, allowing Ca2+ to cross cell membranes.
A23187, free acid is a Ca2+ ionophore. In rat Kupffer cells, A23187 induced hydrolysis of phosphoinositides to inositol phosphates and released inositol phosphates from the cells in a concentration- and time-dependent way [1]. In HL-60 cells, A23187 increased intracellular Ca2+ levels. Also, A23187 generated reactive oxygen species (ROS) both in intracellular and extracellular and induced apoptotic cell death, which was dependent on mitochondrial permeability transition [2]. In ileal muscle, A23187 (10-5 M) induced initial contraction and small rhythmic contractions with decrease in phosphocreatinine (PCr), ATP and glycogen contents under hypoxic conditions. However, A23187 (10-5 M) induced initial contraction and successive rhythmic contractions with a significant decrease in the ATP and glycogen contents in glucose-free medium [3]. In rat C6 glioma cells that was resistant to ZnCl2 (250 μM), A23187 (100 nM) and Zn2+ (150 μM) significantly induced apoptosis. Also, A23187 (1.9 nM) significantly increased the intracellular mobile Zn2+ content. These results suggested that the apoptosis induced by Zn2+ and A23187 was the result of increased Zn2+ influx induced by A23187 [4].
References:
[1]. Gandhi CR, Harvey SA, Cevallos M, et al. A23187 causes release of inositol phosphates from cultured rat Kupffer cells. Eur J Pharmacol, 2001, 415(1): 13-18.
[2]. Kajitani N, Kobuchi H, Fujita H, et al. Mechanism of A23187-induced apoptosis in HL-60 cells: dependency on mitochondrial permeability transition but not on NADPH oxidase. Biosci Biotechnol Biochem, 2007, 71(11): 2701-2711.
[3]. Nasu T, Nishikawa M. Metabolic dependency of ionophore A23187-induced contraction of ileal longitudinal smooth muscle. J Auton Pharmacol, 2000, 20(2): 99-109.
[4]. Jansen S, Arning J, Beyersmann D. Effects of the Ca ionophore a23187 on zinc-induced apoptosis in C6 glioma cells. Biol Trace Elem Res, 2003, 96(1-3): 133-142.
Average Rating: 5
(Based on Reviews and 16 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *