ATPγS tetralithium salt (Synonyms: ATPγS) |
Catalog No.GC12574 |
ATPγS (sel tétralithium) est un substrat pour les activités d'hydrolyse des nucléotides et de déroulement de l'ARN du facteur d'initiation de la traduction eucaryote eIF4A.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 93839-89-5
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
L'adénosine 5'-(γ-thio)-triphosphate (sel de lithium) est un analogue stable de l'ATP qui agit comme un puissant agoniste des récepteurs couplés aux protéines G P2Y2 et P2Y11 (pEC50 = 5,52 pour P2Y11).[1] Il a été utilisé pour identifier les substrats de kinase, a été mis en œuvre en tant que réactif dans la synthèse d'analogues d'acétylglucosamine d'ADN et peut servir de substrat pour les activités d'hydrolyse nucléotidique stimulée par l'ARN et de déroulement de l'ARN du facteur d'initiation eucaryote-4A.[2],[3],[4]
References:
[1]. Jacobson, K.A., Ivanov, A.A., de Castro, S., et al. Development of selective agonists and antagonists of P2Y receptors. Purinergic Signal. 5(1), 75-89 (2009).
[2]. Knowles, J.R. Enzyme-catalyzed phosphoryl transfer reactions. Annual Reviews of Biochemistry 49, 877-919 (1980).
[3]. Chrysogelos, S., Register, J.C., III, and Griffith, J. The structure of recA protein-DNA filaments. 2 recA protein monomers unwind 17 base pairs of DNA by 11.5 degrees/base pair in the presence of adenosine 5'-O-(3-thiotriphosphate). The Journal of Biological Chemisty 258(20), 12624-12631 (1983).
[4]. Peck, M.L., and Herschlag, D. Adenosine 5'-O-(3-thio)triphosphate (ATPγS) is a substrate for the nucleotide hydrolysis and RNA unwinding activities of eukaryotic translation initiation factor elF4A. RNA 9(10), 1180-1187 (2003).
Cas No. | 93839-89-5 | SDF | |
Synonymes | ATPγS | ||
Chemical Name | P'-anhydride with phosphorothioic acid adenosine 5'-(trihydrogen diphosphate), tetralithium salt | ||
Canonical SMILES | S=P([O-])([O-])O[P@](O[P@](OC[C@H]1O[C@@H]([C@@H]([C@@H]1O)O)N2C3=NC=NC(N)=C3N=C2)([O-])=O)([O-])=O.[Li+].[Li+].[Li+].[Li+] | ||
Formula | C10H12Li4N5O12P3S | M.Wt | 546.98 |
Solubility | 10mg/mL in PBS, pH 7.2 | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.8282 mL | 9.1411 mL | 18.2822 mL |
5 mM | 0.3656 mL | 1.8282 mL | 3.6564 mL |
10 mM | 0.1828 mL | 0.9141 mL | 1.8282 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 8 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *