Autophagic Flux Assay Kit

Autophagy is the process of autonomous degradation of cytoplasmic components such as proteins and organelles. Studies have found that it plays an important role in the study of aging, neurodegenerative diseases, etc. As research deepens, many researchers need to confirm the relationship between the autophagic process and the induction of autophagy or the inhibition of autophagic lysosomes, so the study of autophagic flux is crucial.

This kit contains autophagosome and autophagic lysosome detection dye (DAPRed), autophagosome detection dye (DALGreen) and lysosomal acidification inhibitor (bafilomycin A1), which can accurately detect the early autophagic process, autophagic lysosomes and the final digestion and degradation process. ^[1]

This kit contains (DAPRed - Autophagy Detection) for detecting autophagosomes and autophagic lysosomes, (DALGreen - Autophagy Detection) for detecting autophagic lysosomes and lysosomal acidification inhibitor Bafilomycin A1. By adding these reagents, you can experiment the entire process from autophagosome formation to autophagic lysosomes. ^[2][3]

References:
[1] H. Sakurai, et al., "Development of small fluorescent probes for the analysis of autophagy kinetics“, iScience, 2023, 26, 107218.
[2] X. Chen, et al, "Chaiqi decoction ameliorates vascular endothelial injury in metabolic syndrome by upregulating autophagy, Am J Transl Res., 2020, 12(9):4902-4922.
[3] C. Oh, et al, "S-Nitrosylation of p62 Inhibits Autophagic Flux to Promote α-Synuclein Secretion and Spread in Parkinson's Disease and Lewy Body Dementia, J Neurosci., 2022, 42(14), 3011-3024.