C6 NBD Ceramide (d18:1/6:0) (Synonyms: C6 NBD Ceramide, NBD Cer(18:1/2:0), NBD Ceramide (d18:1/6:0) , N-C6-NBD-D-erythro-Sphingosine) |
Catalog No.GC43096 |
C6 NBD Ceramide (d18:1/6:0) est une sonde fluorescente pour appareil de Golgi avec perméabilité de la membrane cellulaire.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 94885-02-6
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
C6 NBD ceramide is a biologically active fluorescent analog of short chain, membrane-permeable ceramides.1 It is as effective as C6 ceramide in the inhibition of viral glycoprotein transport through the Golgi.[1] C6 NBD ceramide has been used as a fluorescent substrate for the activity of UDP-glucose:ceramide glucosyltransferase and to demonstrate the translocation of glucocerebroside and sphingomyelin from the Golgi to the plasma membrane.[2],[3]
Reference:
[1]. Rosenwald, A.G., and Pagano, R.E. Inhibition of glycoprotein traffic through the secretory pathway by ceramide. J. Biol. Chem. 268(7), 4577-4579 (1993).
[2]. Paul, P., Kamisaka, Y., Marks, D.L., et al. Purification and characterization of UDP-glucose: Ceramide glucosyltransferase from rat liver Golgi membranes. J. Biol. Chem. 271(4), 2287-2293 (1996).
[3]. Lipsky, N.G., and Pagano, R.E. Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: Endogenously synthesized sphingomyelin and glucocerebroside analogues pass through the golgi apparatus en route to the plasma membrane. J. Cell. Biol. 100(1), 27-34 (1985).
Cas No. | 94885-02-6 | SDF | |
Synonymes | C6 NBD Ceramide, NBD Cer(18:1/2:0), NBD Ceramide (d18:1/6:0) , N-C6-NBD-D-erythro-Sphingosine | ||
Chemical Name | N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine | ||
Canonical SMILES | CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCCN(C1=CC=C([N+]([O-])=O)C2=NON=C12)[H])=O)CO | ||
Formula | C30H49N5O6 | M.Wt | 575.7 |
Solubility | 5mg/mL in ethanol; 10mg/mL in DMSO; 25mg/mL in DMF | Storage | Store at -20°C; protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.737 mL | 8.6851 mL | 17.3702 mL |
5 mM | 0.3474 mL | 1.737 mL | 3.474 mL |
10 mM | 0.1737 mL | 0.8685 mL | 1.737 mL |
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 15 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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