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C6 NBD Ceramide (d18:1/6:0) (Synonyms: C6 NBD Ceramide, NBD Cer(18:1/2:0), NBD Ceramide (d18:1/6:0) , N-C6-NBD-D-erythro-Sphingosine)

Catalog No.GC43096

C6 NBD Ceramide (d18:1/6:0) est une sonde fluorescente pour appareil de Golgi avec perméabilité de la membrane cellulaire.

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C6 NBD Ceramide (d18:1/6:0) Chemical Structure

Cas No.: 94885-02-6

Taille Prix Stock Qté
1mg
248,00 $US
En stock
5mg
1 119,00 $US
En stock
10mg
1 987,00 $US
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

C6 NBD ceramide is a biologically active fluorescent analog of short chain, membrane-permeable ceramides.1 It is as effective as C6 ceramide in the inhibition of viral glycoprotein transport through the Golgi.[1] C6 NBD ceramide has been used as a fluorescent substrate for the activity of UDP-glucose:ceramide glucosyltransferase and to demonstrate the translocation of glucocerebroside and sphingomyelin from the Golgi to the plasma membrane.[2],[3]

Reference:
[1]. Rosenwald, A.G., and Pagano, R.E. Inhibition of glycoprotein traffic through the secretory pathway by ceramide. J. Biol. Chem. 268(7), 4577-4579 (1993).
[2]. Paul, P., Kamisaka, Y., Marks, D.L., et al. Purification and characterization of UDP-glucose: Ceramide glucosyltransferase from rat liver Golgi membranes. J. Biol. Chem. 271(4), 2287-2293 (1996).
[3]. Lipsky, N.G., and Pagano, R.E. Intracellular translocation of fluorescent sphingolipids in cultured fibroblasts: Endogenously synthesized sphingomyelin and glucocerebroside analogues pass through the golgi apparatus en route to the plasma membrane. J. Cell. Biol. 100(1), 27-34 (1985).

Chemical Properties

Cas No. 94885-02-6 SDF
Synonymes C6 NBD Ceramide, NBD Cer(18:1/2:0), NBD Ceramide (d18:1/6:0) , N-C6-NBD-D-erythro-Sphingosine
Chemical Name N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
Canonical SMILES CCCCCCCCCCCCC/C=C/[C@@H](O)[C@@H](NC(CCCCCN(C1=CC=C([N+]([O-])=O)C2=NON=C12)[H])=O)CO
Formula C30H49N5O6 M.Wt 575.7
Solubility 5mg/mL in ethanol; 10mg/mL in DMSO; 25mg/mL in DMF Storage Store at -20°C; protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.737 mL 8.6851 mL 17.3702 mL
5 mM 0.3474 mL 1.737 mL 3.474 mL
10 mM 0.1737 mL 0.8685 mL 1.737 mL
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Average Rating: 5 ★★★★★ (Based on Reviews and 15 reference(s) in Google Scholar.)

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