(-)-Epigallocatechin gallate (EGCG) (Synonyms: EGCG) |
| Catalog No.GC14049 |
(-)-Epigallocatechin gallate (EGCG) est un polyphénol majeur du thé vert qui inhibe la prolifération cellulaire et induit l'apoptose.
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Cas No.: 989-51-5
Sample solution is provided at 25 µL, 10mM.
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(-)-Epigallocatechin gallate (EGCG) est un polyphénol majeur du thé vert qui inhibe la prolifération cellulaire et induit l'apoptose[1-2]. L'EGCG possède des propriétés de piégeage des radicaux libres[3]. Il exerce également des effets anti-inflammatoires et anti-apoptotiques contre le stress oxydatif dans les neurones[4]. De plus, il inhibe l'activité de la glutamate déshydrogénase 1/2 (GDH1/2, GLUD1/2) [5].
L'EGCG (200 µM ; 1 h) diminue les niveaux d'expression des cytokines pro-inflammatoires et des médiateurs dans les cellules BV2 traitées au CoCl2[6]. L'EGCG inhibe la croissance des cellules 3T3-L1 via la voie miR-143/MAPK7[7].
L'EGCG (5-30 mg/kg ; par voie intragastrique ; 14 jours) réduit la croissance tumorale (cancer colorectal HT-29)[8]. Un traitement à l'EGCG (50 mg/kg par jour) dissous dans de l'huile de maïs administré par injection intrapéritonéale du jour 7 au jour 11, 1 h avant le défi des acariens de la poussière domestique, soulage les symptômes asthmatiques chez les souris en supprimant l'orientation M2 des macrophages médiée par HIF-1α/VEGFA[9].
References:
[1]. Jin CF, Shen SR Sr, et,al. Different effects of five catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. J Agric Food Chem. 2001 Dec;49(12):6033-8. doi: 10.1021/jf010903r. PMID: 11743804.
[2]. De Amicis F, Perri A, et,al. Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Oct;228(10):2054-62. doi: 10.1002/jcp.24372. PMID: 23553645.
[3]. Kondo K, Kurihara M, et,al. Scavenging mechanisms of (-)-epigallocatechin gallate and (-)-epicatechin gallate on peroxyl radicals and formation of superoxide during the inhibitory action. Free Radic Biol Med. 1999 Oct;27(7-8):855-63. doi: 10.1016/s0891-5849(99)00133-1. PMID: 10515590.
[4]. Schroeder EK, Kelsey NA, et,al. Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons. Antioxid Redox Signal. 2009 Mar;11(3):469-80. doi: 10.1089/ars.2008.2215. PMID: 18754708.
[5]. Peeters TH, Lenting K, et,al. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019 May 20;7:4. doi: 10.1186/s40170-019-0198-7. PMID: 31139406; PMCID: PMC6526618.
[6]. Kim SR, Seong KJ, et,al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-κB Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549.
[7]. Chen CP, Su TC, et,al. Green tea epigallocatechin gallate suppresses 3T3-L1 cell growth via microRNA-143/MAPK7 pathways. Exp Biol Med (Maywood). 2022 Sep;247(18):1670-1679. doi: 10.1177/15353702221108925. Epub 2022 Jul 27. PMID: 35894140; PMCID: PMC9597208.
[8]. Jin H, Gong W, et,al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123.
[9]. Yang N, Li X. Epigallocatechin gallate relieves asthmatic symptoms in mice by suppressing HIF-1α/VEGFA-mediated M2 skewing of macrophages. Biochem Pharmacol. 2022 Aug;202:115112. doi: 10.1016/j.bcp.2022.115112. Epub 2022 May 29. PMID: 35640712.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules BV2 |
Méthode de préparation | Les cellules ont été traitées avec de l'EGCG (200 µM) pendant 1 h avant une exposition au CoCl2 (350 µM) pendant 8 h. |
Conditions de réaction | 200 µM ; 1 h |
Domaines d'application | L'(-)-Épigallocatéchine gallate (EGCG) diminue les niveaux d'expression des cytokines pro-inflammatoires et des médiateurs dans les cellules BV2 traitées au CoCl2. |
| Expériences animales [2]: | |
Modèles animaux | Souris BALB/c nues (porteuses du cancer colorectal HT-29) |
Méthode de préparation | Dans le groupe thérapeutique, 5-20 mg/kg d'EGCG ont été administrés par voie intragastrique, et dans le groupe de contrôle, 100 µL de solution saline physiologique ont été administrés par voie intragastrique, une fois par jour pendant 14 jours. |
Forme de dosage | 5-30 mg/kg ; par voie intragastrique ; 14 jours |
Domaines d'application | L'(-)-Épigallocatéchine gallate (EGCG) réduit la croissance tumorale. |
Références : [1]. Kim SR, Seong KJ, et al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-κB Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549. [2]. Jin H, Gong W, et al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123. | |
| Cas No. | 989-51-5 | SDF | |
| Synonymes | EGCG | ||
| Chemical Name | [(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate | ||
| Canonical SMILES | C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O | ||
| Formula | C22H18O11 | M.Wt | 458.37 |
| Solubility | ≥ 22.9mg/mL in DMSO; ≥ 20mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1816 mL | 10.9082 mL | 21.8164 mL |
| 5 mM | 0.4363 mL | 2.1816 mL | 4.3633 mL |
| 10 mM | 0.2182 mL | 1.0908 mL | 2.1816 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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