(-)-Epigallocatechin gallate (EGCG) (Synonyms: EGCG) |
| Catalog No.GC14049 |
(-)-Epigallocatechin gallate (EGCG) est un polyphénol majeur du thé vert qui inhibe la prolifération cellulaire et induit l'apoptose.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 989-51-5
Sample solution is provided at 25 µL, 10mM.
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(-)-Epigallocatechin gallate (EGCG) est un polyphénol majeur du thé vert qui inhibe la prolifération cellulaire et induit l'apoptose[1-2]. EGCG possède des propriétés piégeuses de radicaux libres[3] et exerce des effets anti-inflammatoires et anti-apoptotiques contre le stress oxydatif dans les neurones[4]. De plus, il inhibe l'activité de la glutamate déshydrogénase1/2 (GDH1/2, GLUD1/2) [5].
(-)- Epigallocatechin Gallate(EGCG) (200 µM; 1 h) diminue les niveaux d'expression des cytokines pro-inflammatoires et des médiateurs dans les cellules BV2 traitées au CoCl2[6]. Il inhibe également la croissance des cellules 3T3-L1 via la voie miR-143/MAPK7 [7].
L'administration d'EGCG (5 -30 mg/kg; voie orale; 14 jours) réduit la croissance tumorale (cancer colorectal HT-29)[8]. Un traitement à l'EGCG(50 mg/kg par jour, dissous dans de l'huile de maïs et administré par injection intrapéritonéale du jour 7 au 11, 1 h avant l'exposition aux acariens Dermatophagoïdes)atténue les symptômes asthmatiques chez la souris en supprimant le biais M2 des macrophages médiés par HIF-1α/VEGFA[9].
References:
[1]. Jin CF, Shen SR Sr, et,al. Different effects of five catechins on 6-hydroxydopamine-induced apoptosis in PC12 cells. J Agric Food Chem. 2001 Dec;49(12):6033-8. doi: 10.1021/jf010903r. PMID: 11743804.
[2]. De Amicis F, Perri A, et,al. Epigallocatechin gallate inhibits growth and epithelial-to-mesenchymal transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Oct;228(10):2054-62. doi: 10.1002/jcp.24372. PMID: 23553645.
[3]. Kondo K, Kurihara M, et,al. Scavenging mechanisms of (-)-epigallocatechin gallate and (-)-epicatechin gallate on peroxyl radicals and formation of superoxide during the inhibitory action. Free Radic Biol Med. 1999 Oct;27(7-8):855-63. doi: 10.1016/s0891-5849(99)00133-1. PMID: 10515590.
[4]. Schroeder EK, Kelsey NA, et,al. Green tea epigallocatechin 3-gallate accumulates in mitochondria and displays a selective antiapoptotic effect against inducers of mitochondrial oxidative stress in neurons. Antioxid Redox Signal. 2009 Mar;11(3):469-80. doi: 10.1089/ars.2008.2215. PMID: 18754708.
[5]. Peeters TH, Lenting K, et,al. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019 May 20;7:4. doi: 10.1186/s40170-019-0198-7. PMID: 31139406; PMCID: PMC6526618.
[6]. Kim SR, Seong KJ, et,al. Epigallocatechin Gallate Protects against Hypoxia-Induced Inflammation in Microglia via NF-κB Suppression and Nrf-2/HO-1 Activation. Int J Mol Sci. 2022 Apr 4;23(7):4004. doi: 10.3390/ijms23074004. PMID: 35409364; PMCID: PMC8999549.
[7]. Chen CP, Su TC, et,al. Green tea epigallocatechin gallate suppresses 3T3-L1 cell growth via microRNA-143/MAPK7 pathways. Exp Biol Med (Maywood). 2022 Sep;247(18):1670-1679. doi: 10.1177/15353702221108925. Epub 2022 Jul 27. PMID: 35894140; PMCID: PMC9597208.
[8]. Jin H, Gong W, et,al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53. doi: 10.2147/OTT.S40914. Epub 2013 Mar 8. PMID: 23525843; PMCID: PMC3596123.
[9]. Yang N, Li X. Epigallocatechin gallate relieves asthmatic symptoms in mice by suppressing HIF-1α/VEGFA-mediated M2 skewing of macrophages. Biochem Pharmacol. 2022 Aug;202:115112. doi: 10.1016/j.bcp.2022.115112. Epub 2022 May 29. PMID: 35640712.
| Expériences cellulaires [1]: | |
Lignées cellulaires | Cellules BV2 |
Méthode de préparation | Les cellules ont été traitées avec d'EGCG (200 µM) pendant 1 heure avant une exposition au CoCl2 (350 µM) pendant 8 heures. |
Conditions de réaction | 200 µM; 1 h |
Domaines d'application | (-)- Epigallocatechin Gallate(EGCG) downrégule les niveaux d'expression des cytokines pro-inflammatoires et des médiateurs dans les cellules BV2 traitées au CoCl2. |
| Expériences animales [2]: | |
Modèles animaux | Des souris nude BALB/c (porteuses d'un cancer colorectal HT-29) |
Méthode de préparation | Dans le groupe thérapeutique, une dose de 5 à 20 mg/kg d'EGCG a été administrée par voie intragastrique, tandis que le groupe témoin a reçu 100 uL de solution saline physiologique par même voie, une fois par jour pendant 14 jours. |
Forme de dosage | 5 -30 mg/kg; i.g; 14 jours |
Domaines d'application | (-)- Epigallocatechin Gallate(EGCG) réduit la croissance tumorale. |
References: | |
| Cas No. | 989-51-5 | SDF | |
| Synonymes | EGCG | ||
| Chemical Name | [(2R,3R)-5,7-dihydroxy-2-(3,4,5-trihydroxyphenyl)-3,4-dihydro-2H-chromen-3-yl] 3,4,5-trihydroxybenzoate | ||
| Canonical SMILES | C1C(C(OC2=CC(=CC(=C21)O)O)C3=CC(=C(C(=C3)O)O)O)OC(=O)C4=CC(=C(C(=C4)O)O)O | ||
| Formula | C22H18O11 | M.Wt | 458.37 |
| Solubility | ≥ 22.9mg/mL in DMSO; ≥ 20mg/mL in Water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1816 mL | 10.9082 mL | 21.8164 mL |
| 5 mM | 436.3 μL | 2.1816 mL | 4.3633 mL |
| 10 mM | 218.2 μL | 1.0908 mL | 2.1816 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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