Name: Aminooxyacetic Acid
Brand: GlpBio
SKU: GC45380
Availability: InStock
Rating: 5 (1 reviews)

GlpBio Products Cited In Reputable Papers

Nature
610.7931 (2022): 366-372

Nature
618, 1017–1023 (2023)

Cell
183.7 (2020): 1867-1883

Cell Research
31, 1291–1307 (2021)

Cell Research
33, 273–287 (2023)

Cell Research
33, 546–561 (2023)

Molecular Cancer
21.1 (2022): 1-17

Molecular Cancer
21.1 (2022): 1-18

Cancer Cell
39 (2021): 1-16

Cell Host Microbe
30.8 (2022): 1124-1138

Cell Host Microbe
31.5 (2023): 766-780

Cell Host Microbe
31.3 (2023): 418-43

Cell Metabolism
34.2 (2022): 240-255

Ann Rheum Dis
82(4): 533-545 (2023)

Cell Mol Immunol
20, 264–276 (2023)

Adv Funct Mater
33.35 (2023): 2214998

Product Documents

Quality Control & SDS

View current batch:

Purity: >98.00%

Protocol

Kinase experiment [1]:
Preparation Method	Each reaction was performed in the presence of 100 μL sodium phosphate buffer containing 5 μg of CSE, 10 μM PLP and 1 mM of L-cys as substrates. Inhibitors( Aminooxyacetic Acid) were added 15 min before the L-cys at the indicated concentration and reactions allowed to proceed for 60 min.
Reaction Conditions	10^-7M - 10^-4M Aminooxyacetic Acid at 37℃ for 60 min
Applications	Aminooxyacetic Acid , a frequently used selective CBS inhibitor, also inhibited CSE with an IC50 of 1.1 μM.
Cell experiment [2]:
Cell lines	HCT116 and HT29 cells
Preparation Method	To obtain a concentration of Aminooxyacetic Acid that inhibited cellular H2S synthesis but was noncytotoxic to cell survival, cells were treated with gradient concentrations of Aminooxyacetic Acid for 48 hours. After determining the Aminooxyacetic Acid concentrations, cells were treated with gradient concentrations of OXA in the presence or absence of this specific concentration of Aminooxyacetic Acid for 48 hours, and the IC50 values of OXA were measured.
Reaction Conditions	100 and 200 µmol/L Aminooxyacetic Acid for 48 hours
Applications	Aminooxyacetic Acid inhibited survival of colon cancer cell lines in a concentration-dependent manner, Aminooxyacetic Acid significantly decreased the IC50 values of OXA in HCT116 and HT29 cells.
Animal experiment [3]:
Animal models	Sixty 2-month-old, male, Sprague-Dawley rats weighing 120–160 g
Preparation Method	To establish a model of chronic alcoholism, the model and Aminooxyacetic Acid remedy groups were given free access to 6% (v/v) alcohol solution for 28 days, with the alcohol solution being changed at 9:00 a.m. every day. From day 15 to day 28, rats in the Aminooxyacetic Acid remedy group were intraperitoneally injected once a day with 5 mg/kg Aminooxyacetic Acid dissolved in 1 mL of saline
Dosage form	5 mg/kg Aminooxyacetic Acid from day 15 to day 28
Applications	Aminooxyacetic acid can improve learning and memory in a chronic alcoholism rat model.
References: [1]. Asimakopoulou A, Panopoulos P, Chasapis CT, Coletta C, Zhou Z, Cirino G, Giannis A, Szabo C, Spyroulias GA, Papapetropoulos A. Selectivity of commonly used pharmacological inhibitors for cystathionine β synthase (CBS) and cystathionine γ lyase (CSE). Br J Pharmacol. 2013 Jun;169(4):922-32. doi: 10.1111/bph.12171. PMID: 23488457; PMCID: PMC3687671. [2].Yue T, Zuo S, et,al. Aminooxyacetic acid (AOAA) sensitizes colon cancer cells to oxaliplatin via exaggerating apoptosis induced by ROS. J Cancer. 2020 Jan 20;11(7):1828-1838. doi: 10.7150/jca.35375. PMID: 32194794; PMCID: PMC7052847. [3].Du AL, Qin HZ, et,al. Aminooxyacetic acid improves learning and memory in a rat model of chronic alcoholism. Neural Regen Res. 2018 Sep;13(9):1568-1574. doi: 10.4103/1673-5374.237120. PMID: 30127117; PMCID: PMC6126113.

Aminooxyacetic Acid, an inhibitor of GABA-t, is also inhibited CBS and CSE with an IC50s of 8.5 and 1.1 µM.

The CCK-8 assay indicated that Aminooxyacetic Acid inhibited the viability of colon cancer cells in a concentration-dependent manner. Aminooxyacetic Acid (100 and 200 mol/L) had no effect on the survival of HCT116 and HT29 cells, respectively ^[1]. Aminooxyacetic Acid also decreased the glycolytic rate and the levels of extracellular lactate and pyruvate, without affecting the mitochondrial membrane potential of C6 cells^[2]. Aminooxyacetic acid inhibits the malate-aspartate shuttle in isolated nerve terminals and prevents the mitochondria from utilizing glycolytic substrates^[3].

Aminooxyacetic acid can improve learning and memory in a chronic alcoholism rat model, which may be associated with reduction of hippocampal H2S level and mitochondrial ATPase activity, and up-regulation of myelin basic protein levels in the hippocampus ^[4]. TCE decreased fetal weight, and this was prevented by Aminooxyacetic Acid but not NAC pre/co-treatment to TCE. Although Aminooxyacetic Acid inhibited CCBL activity in maternal kidney, it did not inhibit CCBL activity in maternal liver and placenta, suggesting that Aminooxyacetic Acid prevention of TCE-induced decreased fetal weight was due to CCBL activity inhibition in the kidneys but not liver or placenta ^[5]. Tumor (HCT116 cells) growth was significantly reduced by 9 mg/kg/day Aminooxyacetic Acid ^[6]. Aminooxyacetic acid induced accumulation of GABA in the rat brain ^[7]. Both H2S biosynthesis inhibition (using Aminooxyacetic Acid) and H2S donation (using AP39) suppresses inflammatory mediator production and reduces multi-organ injury in a murine model of burn injury, both at an early time point and at a later time point ^[8]. Aminooxyacetic Acid inhibited growth of SUM159, SUM149, and MCF-7 xenografts and c-myc-overexpressing transgenic mouse mammary tumors ^[9].

References:
[1]. Yue T, Zuo S, et,al. Aminooxyacetic acid (AOAA) sensitizes colon cancer cells to oxaliplatin via exaggerating apoptosis induced by ROS. J Cancer. 2020 Jan 20;11(7):1828-1838. doi: 10.7150/jca.35375. PMID: 32194794; PMCID: PMC7052847.
[2]. Wang C, Chen H, et,al. Malate-aspartate shuttle inhibitor aminooxyacetic acid leads to decreased intracellular ATP levels and altered cell cycle of C6 glioma cells by inhibiting glycolysis. Cancer Lett. 2016 Aug 1;378(1):1-7. doi: 10.1016/j.canlet.2016.05.001. Epub 2016 May 6. PMID: 27157912.
[3]. Kauppinen RA, Sihra TS, et,al. Aminooxyacetic acid inhibits the malate-aspartate shuttle in isolated nerve terminals and prevents the mitochondria from utilizing glycolytic substrates. Biochim Biophys Acta. 1987 Sep 14;930(2):173-8. doi: 10.1016/0167-4889(87)90029-2. PMID: 3620514.
[4]. Du AL, Qin HZ, et,al. Aminooxyacetic acid improves learning and memory in a rat model of chronic alcoholism. Neural Regen Res. 2018 Sep;13(9):1568-1574. doi: 10.4103/1673-5374.237120. PMID: 30127117; PMCID: PMC6126113.
[5]. Su AL, Lash LH, et,al. N-Acetyl-L-cysteine and aminooxyacetic acid differentially modulate trichloroethylene reproductive toxicity via metabolism in Wistar rats. Arch Toxicol. 2021 Apr;95(4):1303-1321. doi: 10.1007/s00204-021-02991-8. Epub 2021 Feb 18. PMID: 33599830; PMCID: PMC8035313.
[6]. Chao C, Zatarain JR,et,al. Cystathionine-beta-synthase inhibition for colon cancer: Enhancement of the efficacy of aminooxyacetic acid via the prodrug approach. Mol Med. 2016 Sep;22:361-379. doi: 10.2119/molmed.2016.00102. Epub 2016 May 16. PMID: 27257787; PMCID: PMC5072412.
[7]. Pagliusi SR, Gomes C, et,al. Aminooxyacetic acid induced accumulation of GABA in the rat brain. Interaction with GABA receptors and distribution in compartments. Naunyn Schmiedebergs Arch Pharmacol. 1983 Apr;322(3):210-5. doi: 10.1007/BF00500767. PMID: 6306485.
[8]. Ahmad A, Szabo C. Both the H2S biosynthesis inhibitor aminooxyacetic acid and the mitochondrially targeted H2S donor AP39 exert protective effects in a mouse model of burn injury. Pharmacol Res. 2016 Nov;113(Pt A):348-355. doi: 10.1016/j.phrs.2016.09.013. Epub 2016 Sep 14. PMID: 27639598.
[9]. Korangath P, Teo WW, et,al.Targeting Glutamine Metabolism in Breast Cancer with Aminooxyacetate. Clin Cancer Res. 2015 Jul 15;21(14):3263-73. doi: 10.1158/1078-0432.CCR-14-1200. Epub 2015 Mar 26. PMID: 25813021; PMCID: PMC4696069.

Cas No.	645-88-5	SDF
Synonymes	Aminooxyacetate
Chemical Name	2-(aminooxy)-acetic acid
Canonical SMILES	OC(CON)=O
Formula	C2H5NO3	M.Wt	91.1
Solubility	5mg/mL in DMSO, 2mg/mL in DMF	Storage	Store at -20°C, protect from light
General tips	Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition	Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
		1 mg	5 mg	10 mg
	1 mM	10.9769 mL	54.8847 mL	109.7695 mL
	5 mM	2.1954 mL	10.9769 mL	21.9539 mL
	10 mM	1.0977 mL	5.4885 mL	10.9769 mL

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Aminooxyacetic Acid (Synonyms: Aminooxyacetate)

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GlpBio Products Cited In Reputable Papers

Product Documents

Quality Control & SDS

Protocol

Complete Stock Solution Preparation Table

Molarity Calculator

Dilution Calculator

In vivo Formulation Calculator (Clear solution)

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