Diolein (Synonyms: Dioleoylglycerol) |
| Catalog No.GC45432 |
Diolein est un type de diacylglycérol (DAG), ce qui est composé de glycérol et de deux molécules d'acide oléique liées par liaisons esters.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 25637-84-7
Sample solution is provided at 25 µL, 10mM.
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Diolein est un type de diacylglycérol (DAG), ce qui est composé de glycérol et de deux molécules d'acide oléique liées par liaisons esters. DAG peut fonctionner en tant qu'un messager seconde dans les voies variées de signalisation cellulaire, particulièrement dans l'activation de la protéine kinase C (PKC)[1][2]. Diolein est largement utilisé dans la recherche biologique cellulaire pour étudier la signalisation lipidique[3], l'activité enzymatique[4], et la dynamique membranaire[5], et est un vecteur non - viral qui a l'espérance de la réussite pour la livraison in vivo de gènes[6].
References:
[1]. McPhail LC, Clayton CC, Snyderman R. A potential second messenger role for arachidonic acid: activation of Ca2+-dependent protein kinase. Trans Assoc Am Physicians. 1984;97:222-31. PMID: 6242084.
[2]. Mosior M, Epand RM. Mechanism of activation of protein kinase C: roles of diolein and phosphatidylserine. Biochemistry. 1993 Jan 12;32(1):66-75. doi: 10.1021/bi00052a010. PMID: 8418861.
[3]. Oishi K, Raynor RL, Charp PA, Kuo JF. Regulation of protein kinase C by lysophospholipids. Potential role in signal transduction. J Biol Chem. 1988 May 15;263(14):6865-71. PMID: 3360811.
[4]. Duan ZQ, Du W, Liu DH. The mechanism of solvent effect on the positional selectivity of Candida antarctica lipase B during 1,3-diolein synthesis by esterification. Bioresour Technol. 2011 Dec;102(23):11048-50. doi: 10.1016/j.biortech.2011.09.003. Epub 2011 Sep 18. PMID: 21978621.
[5]. van Gorkom LC, Nie SQ, Epand RM. Hydrophobic lipid additives affect membrane stability and phase behavior of N-monomethyldioleoylphosphatidylethanolamine. Biochemistry. 1992 Jan 28;31(3):671-7. doi: 10.1021/bi00118a006. PMID: 1731922.
[6]. Guo W, Gosselin MA, Lee RJ. Characterization of a novel diolein-based LPDII vector for gene delivery. J Control Release. 2002 Sep 18;83(1):121-32. doi: 10.1016/s0168-3659(02)00167-0. PMID: 12220844.
| Cas No. | 25637-84-7 | SDF | |
| Synonymes | Dioleoylglycerol | ||
| Canonical SMILES | OC(COC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O | ||
| Formula | C39H72O5 | M.Wt | 621 |
| Solubility | DMF: 10 mg/ml,Ethanol: 10 mg/ml,Ethanol:PBS (pH 7.2) (1:1): 0.5 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.6103 mL | 8.0515 mL | 16.1031 mL |
| 5 mM | 0.3221 mL | 1.6103 mL | 3.2206 mL |
| 10 mM | 0.161 mL | 0.8052 mL | 1.6103 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 1 reference(s) in Google Scholar.)
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