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Gsafe Green plus (10000× in ddH₂O)

Catalog No.GK20003

Gsafe Green plus (equivalent to GelGreen) is an upgraded version of a new type of cyanine nucleic acid dye developed by GlpBio.

Products are for research use only. Not for human use. We do not sell to patients.

Gsafe Green plus (10000× in ddH₂O) Chemical Structure

Taille Prix Stock Qté
500µL
60,00 $US
En stock
2 x 500µL
96,00 $US
En stock
10 x 500µL
350,00 $US
En stock
20 x 500µL
560,00 $US
En stock

Tel:(909) 407-4943 Email: sales@glpbio.com

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Related Biological Data

GelRed

Protocol

Gum dyeing method (front dyeing method)
1. Prepare the agarose gel as usual, add concentrated 10000X Gsafe Green plus to make the final concentration in the gel 1X (for example, prepare 100ml gel, add dye 5μl-10μl, the dosage can be adjusted according to the actual situation), shake gently, pour the glue.
For Marker above 1Kb, it is recommended to reduce the dosage by half.
2. Electrophoresis in accordance with conventional methods, and observe the results.

Bubble dyeing method (post dyeing method)
1. Perform electrophoresis in accordance with conventional methods.
2. Dilute 10000X Gsafe Green plus concentrate with dH2O about 3300 times to 0.1M NaCl to make 3X staining solution. (For example, 15μl 10000X Gsafe Greenplus concentrate and 5ml 1M NaCl were added to 45ml dH2O).
3. Carefully place the gel in a suitable container and slowly add sufficient 3X staining solution to submerge the gel. Shake at room temperature for about 30 minutes. The optimal staining time depends on the gel thickness and agarose, the concentration is slightly different. For 3.5-10% acrylamide glue, the dyeing time is usually between 30min and 1 hour, then observe the results.

Notices
1.Because Gsafe Greenplus has good thermal stability, it can be added directly to the hot agarose solution without waiting for the solution to cool. Shake, oscillate or flip to make sure that the dye is thoroughly mixed. Alternatively, the Gsafe Green plus stock solution can be added to the agarose powder and the electrophoresis buffer, and then heated in a microwave oven or other common methods to prepare agarose gel. Gsafe Green plus is compatible with all commonly used electrophoresis buffer solutions.
2. If you always see band dispersion or separation is not ideal, it is recommended to use bubble dyeing to confirm whether the problem is related to the dye. If the problem persists after dyeing, it means that the problem is the dye is irrelevant, please try: reduce the concentration of agarose; choose a longer gel; extend the gel time to ensure the edge is clear; improve the sampling technique or choose the bubble staining method.
3. Gsafe Green plus has a certain affinity for glassware and non-polypropylene materials. It is recommended to use polypropylene containers in the process of dilution, storage and dyeing.
4. This method is not suitable for prefabricated polyacrylamide gels. For polyacrylamide gels, please use the bubble staining method.

Background

Gsafe Green plus (équivalent à GelGreen) est une version améliorée d'un nouveau type de colorant nucléique cyanine développé par GlpBio. Gsafe Green plus a transformé la matrice cyanine benzène en une chaîne structurée d'huiles macromoléculaires uniques qui ne peuvent pas pénétrer les membranes cellulaires des cellules vivantes et ne sont pas facilement volatilisées ou absorbées par le corps humain, ce qui les rend inutilisables pour la coloration des gels. Il présente des caractéristiques de non-mutagénicité, d'utilisation sûre et non toxique ainsi qu'une détection sensible. En même temps, il améliore les défauts de flexion et de migration des bandes électrophorétiques des colorants nucléiques à base de cyanine et peut être utilisé comme agent de coloration pour diverses électrophorèses d'acides nucléiques, adaptés à la teinture de différentes tailles de fragments. Il est parfaitement compatible avec le système standard d'imagerie UV du gel et l'appareil d'observation du gel excité par la lumière visible bleue, convient aux systèmes d'imagerie UV du gel ou à l'observation sécuritaire par découpe au laser visible bleu, c'est un nouveau colorant nucléique sûr, non toxique et hautement sensible.

Features & Properties

Applications1. Safe and non-toxic: The unique oily macromolecule characteristics make it unable to penetrate the cell membrane and enter the cell. The Ames test result also shows that the mutagenicity of the dye is far less than EB.
2. High sensitivity: It is suitable for electrophoretic staining of fragments of various sizes and has little effect on nucleic acid migration.
3. High stability: It is suitable for preparing agarose gel using microwave or other heating methods; it is extremely stable in acid or alkali buffer at room temperature and has strong light resistance.
4. High signal-to-noise ratio: the sample has a strong fluorescent signal and a low background signal.
5. Simple operation: it does not degrade during precast gel and electrophoresis, and can be directly observed with visible light gel transmission instrument.
6. Wide range of application: you can choose to stain before electrophoresis (gel stain method) or after electrophoresis (bubble stain method); apply to agarose gel or polyacrylamide gel electrophoresis; can be used for dsDNA, ssDNA or RNA staining.
7. Perfect compatibility: suitable for ultraviolet gel imaging system excited by 254nm or gel observation device excited by blue visible light.
ShippingShip with blue ice.
Storage ConditionsStore at 2-8˚C (Avoid direct sunlight), and is stable for up to 2 years.
UsageFor research use only! Not for use on humans.

Related Video

    Gsafe Green plus- GlpBio

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Average Rating: 5 ★★★★★ (Based on Reviews and 31 reference(s) in Google Scholar.)

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