Insulin(cattle) (Insulin from bovine pancreas) |
| Catalog No.GC31303 |
Insulin(cattle) (Insulin from bovine pancreas) est une protéine hormonale composée de deux chaînes de 21 et 30 acides aminés.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11070-73-8
Sample solution is provided at 25 µL, 10mM.
Insulin(cattle) (Insulin from bovine pancreas) est une protéine hormonale composée de deux chaînes de 21 et 30 acides aminés. Insulin agit sur les neurones et les cellules gliales pour réguler le métabolisme glucidique systémique et l'alimentation. Insulins(cattle) est un type d'insuline native. Insulins(cattle) a été utilisée pour traiter les patients avec diabète sucré[1].
La signalisation de l'insuline est initiée par la liaison de l'insuline au récepteur de l'insuline, l'activation du domaine protéine tyrosine kinase et l'autophosphorylation en tyrosine du récepteur de l'insuline, la phosphorylation en tyrosine extensive des protéines de substrats du récepteur de l'insuline (IRS) et la phosphorylation de S473 dans AKT[2]
Les rats qui sont injectés par l'insuline (i.v., 0.5 IU/ kg−1 poids corporel) et la réactivité immunologique d' AKT phosphorylée (pAKT) a été évaluée dans les tancytes. Ces analyses ont révélé que le traitement de l'insuline induisait fortement la phosphorylation d' AKT dans les tancytes des rats de témoin, et que cette activation était largement diminuée dans les tancytes des rats IR∆Tan ( rats dépourvus de récepteurs à l'insuline dans les tancytes)[3]
References:
[1]. Adams GG, Meal A, Morgan PS, Alzahrani QE, Zobel H, Lithgo R, Kok MS, Besong DTM, Jiwani SI, Ballance S, Harding SE, Chayen N, Gillis RB. Characterisation of insulin analogues therapeutically available to patients. PLoS One. 2018 Mar 29;13(3):e0195010.
[2]. Brown M, Dainty S, et al. Endoplasmic reticulum stress causes insulin resistance by inhibiting delivery of newly synthesized insulin receptors to the cell surface. Mol Biol Cell. 2020 Nov 1;31(23):2597-2629.
[3]. Porniece Kumar M, et al. Insulin signalling in tanycytes gates hypothalamic insulin uptake and regulation of AgRP neuron activity. Nat Metab. 2021 Dec;3(12):1662-1679.
References:
[1]. Brown M, Dainty S, et al. Endoplasmic reticulum stress causes insulin resistance by inhibiting delivery of newly synthesized insulin receptors to the cell surface. Mol Biol Cell. 2020 Nov 1;31(23):2597-2629.
[2]. Porniece Kumar M, et al. Insulin signalling in tanycytes gates hypothalamic insulin uptake and regulation of AgRP neuron activity. Nat Metab. 2021 Dec;3(12):1662-1679.
| Cas No. | 11070-73-8 | SDF | |
| Canonical SMILES | Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala. Gly-Ile-Val-Glu-Gln-Cys-Cys-Ala-Ser-Val-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn (Disulfide bridge: Cys7-Cys7', Cys19-Cys20', Cys6' | ||
| Formula | C254H377N65O75S6 | M.Wt | 5733.49 |
| Solubility | Water : 12.5 mg/mL (2.18 mM; adjust pH to 2 with HCl and heat to 60°C) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 174.4 μL | 872.1 μL | 1.7441 mL |
| 5 mM | 34.9 μL | 174.4 μL | 348.8 μL |
| 10 mM | 17.4 μL | 87.2 μL | 174.4 μL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Potency: 26 U/mg
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)
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