L-NIO (hydrochloride) |
| Catalog No.GC10281 |
NOS inhibitor
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 36889-13-1
Sample solution is provided at 25 µL, 10mM.
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IC50: 1.7, 3.9, and 3.9 μM for nNOS (rat), eNOS (bovine), and iNOS (mouse), respectively
L-NIO is a NOS inhibitor.
Nitric oxide synthases (NOSs) are enzymes that catalyze the production of nitric oxide (NO) from L-arginine. NO is an important cellular signaling molecule and helps modulate vascular tone, airway tone, insulin secretion, and peristalsis. NO is involved in angiogenesis and neural development.
In vitro: Three analogues of L-arginine were characterized as inhibitors of endothelial NO synthase by measuring their effect on the endothelial NO synthase from porcine aortae. It was found that L-NMMA, L-NIO and L-NAME could cause concentration-dependent inhibition of the Ca2+-dependent endothelial NO synthase [1].
In vivo: In rats, L-NMMA, L-NIO and L-NAME could induce a dose-dependent increase in mean systemic arterial blood pressure accompanied by bradycardia. Moreover, L-NMMA, L-NIO and L-NAME at i.v. 100 mg/kg significantly inhibited the hypotensive responses to ACh and bradykinin. The increase in blood pressure and bradycardia produced by these compounds were able to be reversed by L-arginine in a dose-dependent manner. Moreover, all of these effects were enantiomer specific [1].
Clinical trial: So far, no clinical study has been conducted.
Reference:
[1] D. D. Rees, R. M. J. Palmer, R. Schulz, et al. Characterization of three inhibitors of endothelial nitric oxide synthase in vitro and in vivo. British Journal of Pharmacology 101, 746-752 (1990).
| Cas No. | 36889-13-1 | SDF | |
| Chemical Name | N5-(1-iminoethyl)-L-ornithine, dihydrochloride | ||
| Canonical SMILES | CC(=N)NCCC[C@H](N)C(=O)O | ||
| Formula | C7H15N3O2 • 2HCl | M.Wt | 246.1 |
| Solubility | ≤20mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 4.0634 mL | 20.3169 mL | 40.6339 mL |
| 5 mM | 0.8127 mL | 4.0634 mL | 8.1268 mL |
| 10 mM | 0.4063 mL | 2.0317 mL | 4.0634 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 26 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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