N-glycolyl-Ganglioside GM3 Mixture (ammonium salt) (Synonyms: GM3-Neugc, NeuGc-GM3, NGcGM3, N-glycolyl-GM3, N-glycolyl-Monosialoganglioside GM3, N-Glycolylneuraminyllactosylceramide) |
| Catalog No.GC48335 |
A mixture of N-glycolyl-ganglioside GM3
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 69345-49-9
Sample solution is provided at 25 µL, 10mM.
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N-glycolyl-Ganglioside GM3 (NeuGc-GM3) is a form of ganglioside GM3 that contains an N-glycolylated sialic acid (NeuGc). It is not biosynthesized in humans under baseline conditions due to a mutation in CMP-Neu5Ac hydroxylase (CMAH), which converts N-acetyl sialic acid to N-glycolyl sialic acid, but NeuGc can be taken up by human cells via the diet and incorporated into glycolipids, such as ganglioside GM3.1,2 NeuGc-GM3 impairs differentiation and maturation of dendritic cells in vitro and reduces CD4 expression in non-activated T cells.1,3 It increases the number of lung tumor nodules formed in a murine Lewis lung carcinoma model.4 It is found in a variety of human cancers, including breast and lung cancers, as well as pediatric cancers.1 Levels of NeuGc-GM3 are negatively correlated with the number of mature dendritic cells in patient-derived non-small cell lung cancer (NSCLC) tumor tissue.5 Anti-NeuGc-GM3 antibodies, able to destroy tumor cells in vitro, have been found in individuals without cancer but not in patients with NSCLC.1,6 N-glycolyl-Ganglioside GM3 mixture contains ganglioside GM3 molecular species with primarily C22:0, C23:0, and C24:0 fatty acyl chain lengths. [Matreya, LLC. Catalog No. 1553]
1.Labrada, M., Dorvignit, D., Hevia, G., et al.GM3(Neu5Gc) ganglioside: An evolution fixed neoantigen for cancer immunotherapySemin. Oncol.45(1-2)41-51(2018) 2.Tangvoranuntakul, P., Gagneux, P., Diaz, S., et al.Human uptake and incorporation of an immunogenic nonhuman dietary sialic acidProc. Natl. Acad. Sci. USA100(21)12045-12050(2003) 3.de LeÓn, J., FernÁndez, A., Clavell, M., et al.Differential influence of the tumour-specific non-human sialic acid containing GM3 ganglioside on CD4+CD25- effector and naturally occurring CD4+CD25+ regulatory T cells functionInt. Immunol.20(4)591-600(2008) 4.Segatori, V.I., Vazquez, A.M., Gomez, D.E., et al.Preclinical evaluation of racotumomab, an anti-idiotype monoclonal antibody to N-glycolyl-containing gangliosides, with or without chemotherapy in a mouse model of non-small cell lung cancerFront. Oncol.2160(2012) 5.van Cruijsen, H., Ruiz, M.G., van der Valk, P., et al.Tissue micro array analysis of ganglioside N-glycolyl GM3 expression and signal transducer and activator of transcription (STAT)-3 activation in relation to dendritic cell infiltration and microvessel density in non-small cell lung cancerBMC Cancer9180(2009) 6.RodrÍguez-Zhurbenko, N., MartÍnez, D., Blanco, R., et al.Human antibodies reactive to NeuGcGM3 ganglioside have cytotoxic antitumor propertiesEur. J. Immunol.43(3)826-837(2013)
| Cas No. | 69345-49-9 | SDF | |
| Synonymes | GM3-Neugc, NeuGc-GM3, NGcGM3, N-glycolyl-GM3, N-glycolyl-Monosialoganglioside GM3, N-Glycolylneuraminyllactosylceramide | ||
| Canonical SMILES | O[C@H]1[C@H](O[C@@H]([C@@H]([C@H]2O[C@]3(C([O-])=O)C[C@@H]([C@H]([C@]([C@@H]([C@H](O)CO)O)(O3)[H])NC(CO)=O)O)O)O[C@@H]([C@@H]2O)CO)[C@@H](CO)O[C@@H](OC[C@@H]([C@H](O)/C=C/CCCCCCCCCCCCC)NC([R])=O)[C@@H]1O.[NH4+] | ||
| Formula | C64H117N2O22•NH4 (for tricosanoyl) | M.Wt | 1284.7 |
| Solubility | Storage | -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7784 mL | 3.892 mL | 7.7839 mL |
| 5 mM | 0.1557 mL | 0.7784 mL | 1.5568 mL |
| 10 mM | 0.0778 mL | 0.3892 mL | 0.7784 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
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