Senktide |
| Catalog No.GC14839 |
Senktide est un agoniste des récepteurs de la tachykinine NK3.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 106128-89-6
Sample solution is provided at 25 µL, 10mM.
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Senktide is a tachykinin NK3 receptor agonist.
The selective NK3 receptor agonist Senktide excites 24 of 31 dopaminergic neurons in the substantia nigra pars compacta in a concentration-dependent manner. The effective concentration range is between 3 to 3000 nm. The mean EC50 for Senktide is 41.2±9 nm (n=5)[2].
I.c.v. injection of Senktide causes a dose-dependent increase in total distance traveled (F6,72=6.344, P<0.001). This increase reaches statistical significance compare to the vehicle-treated group at 0.06 nmol and higher. The Senktide-induced increase in locomotor activity brought about by 0.1 nmol of Senktide is significantly and dose-dependently decreased by the tachykinin NK3 receptor antagonists talnetant at 30 mg/kg and SB222200 at 30 mg/kg, but not by osanetant, when tested in parallel in a single experiment (F7,78=10.32, P<0.001), although a non-significant reduction is observed. However, when tested using another vehicle (Vitamin E and glycofurol), osanetant does decrease activity significantly compare to Senktide-treated gerbils (F2,30=10.10, P<0.001)[1].
References:
[1]. Nordquist RE, et al. The tachykinin NK3 receptor agonist senktide induces locomotor activity in male Mongolian gerbils. Eur J Pharmacol. 2008 Dec 14;600(1-3):87-92.
[2]. Keegan KD, et al. The selective NK3 receptor agonist senktide excites a subpopulation of dopamine-sensitiveneurones in the rat substantia nigra pars compacta in vitro. Br J Pharmacol. 1992 Jan;105(1):3-5.
Cell experiment: | Experiments are performed on brain slices (300 μM thick) from 150 g male Wistar rats and extracellular recordings are made by conventional techniques. Drugs (including Senktide) are applied by bath perfusion and removal is achieved simply by returning to the control drug-free solution. Extracellular electrodes are filled with aCSF and have resistances of 5 to 14 MΩ. For intracellular recordings, electrodes are filled with 1 M potassium acetate and have d.c. resistances of 70 to 110 MΩ. Neurons are considered to be dopaminergic if they have a characteristic waveform, slow firing rate (~5 Hz) and inhibitory response to dopamine[2]. |
Animal experiment: | For the Senktide dose-response curve, gerbils are first allowed to habituate to the test area for 30 min. Animals (n=10 to 12 per drug treatment group) are anesthetized with isoflurane, a small incision is made in the skin over bregma, and an injection of Senktide at 0.01, 0.03, 0.06, 0.1, 0.3 or 0.6 nmol in 5 μL of vehicle is placed i.c.v. using a syringe with a 4.5 mm long needle. Wounds are clipped shut, and animals allowed to awaken from anesthesia, then placed directly into the locomotor activity boxes and recording commenced. For testing of the NK1 receptor antagonist aprepitant (1, 3 or 10 mg/kg p.o.), gerbils are first treated with aprepitant or vehicle (0.9% NaCl with 0.3% Tween80) and returned to the home cage for 90 min. Animals are then placed in the open field for 30 min of habituation. During the last 5 min of habituation, 0.03 nmol Senktide is injected i.c.v[1]. |
References: [1]. Nordquist RE, et al. The tachykinin NK3 receptor agonist senktide induces locomotor activity in male Mongolian gerbils. Eur J Pharmacol. 2008 Dec 14;600(1-3):87-92. | |
| Cas No. | 106128-89-6 | SDF | |
| Chemical Name | (5S,8S,14S,17S,20S)-14,17-dibenzyl-5-carbamoyl-20-(carboxymethyl)-8-isobutyl-15-methyl-7,10,13,16,19,22-hexaoxo-2-thia-6,9,12,15,18,21-hexaazapentacosan-25-oic acid | ||
| Canonical SMILES | O=C([C@H](CC1=CC=CC=C1)NC([C@H](CC(O)=O)NC(CCC(O)=O)=O)=O)N(C)[C@H](C(NCC(N[C@H](C(N[C@H](C(N)=O)CCSC)=O)CC(C)C)=O)=O)CC2=CC=CC=C2 | ||
| Formula | C40H55N7O11S | M.Wt | 841.97 |
| Solubility | DMSO: 5 mg/ml,PBS (pH 7.2): 2 mg/ml | Storage | Desiccate at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.1877 mL | 5.9385 mL | 11.8769 mL |
| 5 mM | 0.2375 mL | 1.1877 mL | 2.3754 mL |
| 10 mM | 0.1188 mL | 0.5938 mL | 1.1877 mL |
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- Purity: >99.00%
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Average Rating: 5 (Based on Reviews and 34 reference(s) in Google Scholar.)
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