Polybrene |
| Catalog No.GC19206 |
Polybrene (Polybrene) is a cationic polymer used routinely to enhance the efficiency of retrovirus vector-mediated gene transfer.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.:28728-55-4
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
| Cell experiment [1]: | |
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Cell lines |
Induced pluripotent stem cell (iPSC)-differentiated neuron system |
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Reaction Conditions |
0-32 µg/mL;24h |
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Applications |
Polybrene displays concentration-dependent effects. Starting at concentrations as low as 4 µg/mL, distinct neuritic beads emerge, whereas a concentration of 8 µg/mL induces neuritic fragmentation. |
| Animal experiment [2]: | |
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Animal models |
Adult (2-3 months) BALB/c mice |
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Preparation Method |
4 µg/µL polybrene was incubated at 37℃ for 15 min, and intracerebroventricularly (ICV) injection of 5 µL mixture was performed for 20 min. Rotating Rod test will take place in three days. |
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Dosage form |
4 µg/µL；ICV |
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Applications |
Polybrene injection resulted in mice exhibiting motor dysfunction on the third day. |
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References: [1]. Bao F, Shi H,et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003. |
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Polybrene, a quaternary ammonium salt, was firstly introduced into clinical practice as a heparin neutralizer[1]. Besides, polybrene at lower doses was popularly used to promote the efficiency of virus-mediated gene transfer both in vivo and in vitro[2-5]. Polybrene promote the binding of viruses on the cell surface by neutralizing the electrostatic repulsion between the opposing bilayers[6-7].
Polybrene(0-32 µg/mL;24h) displays concentration-dependent effects. Starting at concentrations as low as 4 µg/mL, distinct neuritic beads emerge, whereas a concentration of 8 µg/mL induces neuritic fragmentation[8]. Polyethylene(8µg/mL) significantly reduced the proliferation rate of Mesenchymal stem cells (MSCs) [9]. Polybrene markedly enhances the efficiency of lipofection and also compensates the serum-mediated inhibition of lipofection in a variety of cell lines[10].
Polybrene(4 µg/µL；ICV) injection resulted in mice exhibiting motor dysfunction on the third day.Polybrene induces neural death and gliosis in mice[8].
References:
[1]. Weiss.W. A, Gilman, J. S, et,al. Heparin neutralization with polybrene administered intravenously. J. Am. Med. Assoc. 166, 603-607 (1958).
[2]. Davis, H. E., Morgan, J. R. et,al. Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys. Chem. 97, 159-172 (2002).
[3]. Bellodi-Privato, M. et al. AAV gene transfer to the retina does not protect retrovirally transduced hepatocytes from the immune response. J. Mol. Med (Berl.). 82, 403-410 (2004).
[4]. Lauwers, E. et al. Neuropathology and neurodegeneration in rodent brain induced by lentiviral vector-mediated overexpression of alpha-synuclein. Brain Pathol. 13, 364-372 (2003).
[5]. Ferry, N., Duplessis, O., et,al. Retroviral-mediated gene transfer into hepatocytes in vivo. Proc. Natl Acad. Sci. USA 88, 8377-8381 (1991).
[6]. Sinn, P. L., Sauter, S. L. et,al. Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors--design, biosafety, and production. Gene Ther. 12, 1089-1098 (2005).
[7]. Kafri, T. Lentivirus vectors: difficulties and hopes before clinical trials. Curr. Opin. Mol. Ther. 3, 316-326 (2001).
[8]. Bao F, Shi H, et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003.
[9].Lin P, Correa D, et,al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891. doi: 10.1371/journal.pone.0023891. Epub 2011 Aug 26. PMID: 21887340; PMCID: PMC3162604.
[10].Abe A, Miyanohara A, Friedmann T. Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 May;5(5):708-11. doi: 10.1038/sj.gt.3300652. PMID: 9797877.
| Cas No. | 28728-55-4 | SDF | |
| Formula | M.Wt | ||
| Solubility | 10mg/ml in Water | Storage | Store at 2-8℃, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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