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Bovine Serum Albumin (BSA)

Catalog No.GC35542

Bovine Serum Albumin (BSA) Chemical Structure

Size Price Stock Qty
1g
$46.00
In stock
10g
$237.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Quality Control & SDS

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Protocol

Cell experiment:

Samples are prepared in 96-well microplates and all wells without samples are filled with 200 μL PBS to minimize evaporation. To each well is loaded 100 μL SH-SY5Y growth medium with or without 10.000 SH-SY5Y neuroblastoma cells. The assay is started by adding 25 μL buffer, with or without 5 mg/mL sonicated Bovine Serum Albumin previously incubated for 0, 2 or 96 h at 70°C (giving a final BSA concentration of 1 mg/mL). All sample combinations are loaded in triplets, and for each of the six Bovine Serum Albumin combinations a corresponding triplet is loaded without cells, to be used as blank. One triplet is also loaded without both cells and BSA and one only without Bovine Serum Albumin[1].

References:

[1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38.

Background

Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications.

Bovine serum albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. Bovine serum albumin constitutes ca. 60% of all plasma protein and binds and transports a large number of physiological and non-physiological ligands. Bovine serum albumin contains 17 disulfide bonds and one unpaired cysteine (Cys34), which facilitates dimerization and also influences higher-order association, since the rate of aggregation is slowed down if Cys34 is covalently bound to another compound. MTT assay for fibril cytotoxicity shows Bovine Serum Albumin is beneficial for cell growth irrespective of its aggregated state. Lack of cytotoxicity is confirmed by membrane permeabilization assays. In the subsequent 40 h, an increase in the viability of the treated cells of 300-400% is observed, indicating that the cells incubated with Bovine Serum Albumin achieve a higher viability than the untreated cells[1].

References:
[1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38.

Chemical Properties

Cas No. 9048-46-8 SDF
Synonyms N/A
Chemical Name N/A
Canonical SMILES S=B[*]
Formula N/A M.Wt 66.43kDa
Solubility H2O : 100 mg/mL Storage Store at -20°C, protect from light
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 17 reference(s) in Google Scholar.)

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