Ginsenoside Rg1 (Synonyms: Ginsenoside A2, Panaxoside A, Panaxoside Rg1, Sanchinoside C1, Sanchinoside Rg1) |
Catalog No.GN10544 |
Ginsenoside Rg1 is one of the main active ingredients of ginseng and a steroidal glycoside with various biological activities. Ginsenoside Rg1 reduces brain Aβ levels and reduces NF-κB nuclear translocation.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 22427-39-0
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Cell experiment [1]: |
|
Cell lines |
human dental pulp cells |
Preparation method |
hDPCs were incubated with different concentrations of ginsenoside Rg1 (0.1, 0.5, 2.5, 5, 10 and 20 μmol/L). The effects of ginsenoside Rg1 on the proliferative ability of hDPCs were evaluated by a fibroblast colony forming test, MTT assay and flow cytometry for cell cycle. DPCs were incubated with various concentrations of ginsenoside Rg1 for 1, 3, 5 and 7 days. |
Reaction Conditions |
0.1, 0.5, 2.5, 5, 10 and 20 μmol/L;1, 3, 5 and 7 days |
Applications |
Ginsenoside Rg 1 (5 μmol/L) significantly promoted the proliferation and differentiation of human dental pulp cells hDPC. Compared with the control group, ALP activity, DSPP and DMP 1 gene expression were increased in the induction group, ginsenoside Rg 1 group and the two combined groups. |
Animal experiment [2]: |
|
Animal models |
APP/PS1 mice |
Preparation method |
According to the concentration-response curves, the mice in the G-Rg1 and G-Rg2 groups were injected intraperitoneally once daily with G-Rg1 and G-Rg2 (30 mg/kg), respectively, dissolved in saline. The mice in the AD model group (APP/PS1 mice) and the normal control group (C57BL/6J nontransgenic littermates) were treated with isodose saline (0.9% w/v). All mice were treated for 1 month before brain metabolite profiling. |
Dosage form |
30 mg/kg;1 month |
Applications |
Ginsenoside Rg 1 and ginsenoside Rg 2 treatment (30 mg/kg; 1 month) effectively improved the cognitive function of APP/PS1 mice, and a gradual improvement in brain pathological abnormalities was observed, and the accumulation of Aβ deposits was effectively reduced. |
References: [1] Wang P, et al. Effect of ginsenoside Rg1 on proliferation and differentiation of human dental pulp cells in vitro. Aust Dent J. 2012 Jun;57(2):157-65. [2] Li N, et al. A UPLC/MS-based metabolomics investigation of the protective effect of ginsenosides Rg1 and Rg2 in mice with Alzheimer's disease. J Ginseng Res. 2016 Jan;40(1):9-17. |
Ginsenoside Rg1, one of the main active components found in Panax ginseng, is a class of natural products known as steroid glycosides with a wide range of biological activities. Ginsenoside Rg1 has attracted significant attention due to its various pharmacological effects, including the promotion of hippocampal neurogenesis, improvement of neural plasticity, enhancement of learning and memory, anti-aging and anti-fatigue effects, as well as immunoregulatory and anti-tumor activities. Treatment with G-Rg1 has also been shown to significantly reduce the expression of TNF-α, IL-1β, and IL-6 in ischemia-reperfusion animal and cell models[1]. Ginsenoside Rg1 has been demonstrated to have significant neuroprotective effects, reduce brain levels of Aβ, and decrease NF-κB nuclear translocation, making it a potential therapeutic agent for neurodegenerative diseases such as Alzheimer's and Parkinson's diseases[4].
In vitro, ginsenoside Rg1 (5 μmol/L) significantly promoted the proliferation and differentiation of human dental pulp cells (hDPCs). Compared with the control group, the induced group, the ginsenoside Rg1 group, and the combination of both showed increased ALP activity and expressions of DSPP and DMP1 genes[1]. Another study indicated that Rg1 significantly reduced the levels of TNF-α and IL-6 in inflamed joints of AIA rats and LPS-stimulated RAW 264.7 cells, increased PPAR-γ protein expression, and inhibited the phosphorylation of IκBα and the nuclear translocation of NF-κB[2].
In vivo, intraperitoneal injection of Rg1 (5, 10, and 20 mg/kg) in AIA rats for 14 consecutive days showed therapeutic effects on arthritis, significantly alleviating joint swelling and damage[2]. Moreover, treatment with ginsenoside Rg1 and Rg2 (30 mg/kg; 1 month) effectively improved the cognitive function of APP/PS1 mice, observed gradual improvement in brain pathological abnormalities, and effectively reduced the accumulation of Aβ deposits[3].
References:
[1] Zhang L, et al. Ginsenoside Rg1 attenuates adjuvant-induced arthritis in rats via modulation of PPAR-γ/NF-κB signal pathway. Oncotarget. 2017 Jul 24;8(33):55384-55393.
[2] Wang P, et al. Effect of ginsenoside Rg1 on proliferation and differentiation of human dental pulp cells in vitro. Aust Dent J. 2012 Jun;57(2):157-65.
[3] Li N, et al. A UPLC/MS-based metabolomics investigation of the protective effect of ginsenosides Rg1 and Rg2 in mice with Alzheimer's disease. J Ginseng Res. 2016 Jan;40(1):9-17.
[4] Xie W , Zhou P , Sun Y ,et al. Protective Effects and Target Network Analysis of Ginsenoside Rg1 in Cerebral Ischemia and Reperfusion Injury: A Comprehensive Overview of Experimental Studies[J].Cells, 2018, 7(12).
Cas No. | 22427-39-0 | SDF | |
Synonyms | Ginsenoside A2, Panaxoside A, Panaxoside Rg1, Sanchinoside C1, Sanchinoside Rg1 | ||
Chemical Name | Ginsenoside Rg1 | ||
Canonical SMILES | CC(=CCCC(C)(C1CCC2(C1C(CC3C2(CC(C4C3(CCC(C4(C)C)O)C)OC5C(C(C(C(O5)CO)O)O)O)C)O)C)OC6C(C(C(C(O6)CO)O)O)O)C | ||
Formula | C42H72O14 | M.Wt | 801.01 |
Solubility | ≥ 32mg/mL in DMSO | Storage | Store at 4°C Keep from the light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 1.2484 mL | 6.2421 mL | 12.4842 mL |
5 mM | 0.2497 mL | 1.2484 mL | 2.4968 mL |
10 mM | 0.1248 mL | 0.6242 mL | 1.2484 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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