Guanylyl Imidodiphosphate (lithium salt) (Synonyms: Gpp(NH)p lithium) |
| Catalog No.GC43798 |
Guanylyl Imidodiphosphate (lithium salt), a stable GTP analog, is a potent activator of adenylate cyclase.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 64564-03-0
Sample solution is provided at 25 µL, 10mM.
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Guanylyl Imidodiphosphate (lithium salt), a stable GTP analog, is a potent activator of adenylate cyclase[1]. Guanylyl Imidodiphosphate reduces the binding of agonist ligands (±)-[3H]epinephrine and (-)-[3H]norepinephrine to α-noradrenergic receptors in calf frontal cerebral cortex membranes, exhibiting IC50 values with 1-10μM[1]. Guanylyl Imidodiphosphate selectively and potently inhibits the high-affinity binding of the tritiated agonist with an IC50 value of 0.1µM[2-3].
In vitro, Guanylyl Imidodiphosphate and GTP significantly enhanced enzymatic activity of adenylate cyclase (EC4.6.1.1) in the renal plasma membranes of male Ranger strain chicks (Ross Poultry, Worcester)[4-5]. Guanylyl Imidodiphosphate, with EC50 achieved at 3.1μM concentration after 12min incubation, produced an 8.2-fold (720%) enhancement in enzymatic activity of adenylate cyclase relative to basal levels[5]. Guanylyl Imidodiphosphate demonstrated dose-dependent inhibition of nuclear membrane assembly in Xenopus egg extracts, at a concentration of 3mM, Guanylyl Imidodiphosphate completely blocked both vesicle fusion and nuclear envelope formation throughout the 160 minutes. When applied at 1mM concentration, Guanylyl Imidodiphosphate delayed the initiation of vesicle fusion by 80 minutes post-cytosol addition and significantly suppressed nuclear envelope expansion, reducing the growth rate of nuclear envelope by more than 6-fold[6].
References:
[1] U'Prichard D C, Snyder S H. Guanyl nucleotide influences on 3H-ligand binding to α-noradrenergic receptors in calf brain membranes[J]. Journal of Biological Chemistry, 1978, 253(10): 3444-3452.
[2] Mollereau C, Pascaud A, Baillat G, et al. 5′-Guanylylimidodiphosphate decreases affinity for agonists and apparent molecular size of a frog brain opioid receptor in digitonin solution[J]. Journal of Biological Chemistry, 1988, 263(34): 18003-18008.
[3] Roy C, LE BARS N C, JARD S. Vasopressin‐Sensitive Kidney Adenylate Cyclase: Differential Effects of Monovalent Ions on Stimulation by Fluoride, Vasopressin and Guanylyl 5′‐imidodiphosphate[J]. European Journal of Biochemistry, 1977, 78(2): 325-332.
[4] ABOU-ISSA H, REICHERT JR L E O E. Modulation of follicle-stimulating hormone-sensitive rat testicular adenylate cyclase activity by guanyl nucleotides[J]. Endocrinology, 1979, 104(1): 189-193.
[5] Hunt N H, Martin T J, Michelangeli V P, et al. Effect of guanyl nucleotides on parathyroid hormone-responsive adenylate cyclase in chick kidney[J]. Journal of Endocrinology, 1976, 69(3): 401-412.
[6] Boman A L, Delannoy M R, Wilson K L. GTP hydrolysis is required for vesicle fusion during nuclear envelope assembly in vitro[J]. The Journal of cell biology, 1992, 116(2): 281-294.
| Cell experiment [1]: | |
Cell lines | Xenopus egg cells |
Preparation Method | Xenopus egg extracts were prepared through sequential centrifugation steps to isolate cytosolic and membrane fractions. Initially, dejellied Xenopus eggs were subjected to low-speed centrifugation (10000g, 12min) to generate a crude cytoplasmic extract. The supernatant was then fractionated by ultracentrifugation (200000g, 1h, 4°C) to separate membrane and cytosolic components. The cytosolic fraction underwent additional clarification (200000g, 25min) and was supplemented with an ATP-regenerating system (1mM ATP, 50μg/ml creatine phosphokinase, 10mM creatine phosphate) before aliquoting and storage in liquid nitrogen (-80°C). The membrane fraction was purified through a sucrose cushion (1.3M sucrose, 26600g, 15min, 4°C) following dilution in membrane wash buffer (MWB), then resuspended in MWB containing 0.5M sucrose at a concentration equivalent to 10% of the cytosolic volume. Both fractions exhibited protein concentrations ranging from 25-35 mg/ml. For functional assays, thawed membrane fractions were incubated with cytosol and guanylyl imidodiphosphate (lithium salt) (0.1-3mM) for 30-180min at 23°C. Following pre-incubation, samples were diluted with MWB containing Guanylyl Imidodiphosphate (lithium salt) and pelleted (20000g, 15min, 4°C). The treated membranes were resuspended to their original volume in MWB, and 2μl aliquots were combined with 1μl swollen chromatin for 30 minutes binding. Membrane fusion activity was subsequently assessed by adding 20μl fresh cytosol to the reaction mixture. |
Reaction Conditions | 0.1, 1 and 3mM; 60, 120 and 180min |
Applications | Guanylyl Imidodiphosphate (lithium salt) significantly blocked both vesicle fusion and nuclear envelope formation. |
References: | |
| Cas No. | 64564-03-0 | SDF | |
| Synonyms | Gpp(NH)p lithium | ||
| Chemical Name | 5’-guanylic acid, monoanhydride with imidodiphosphoric acid, tetralithium salt | ||
| Canonical SMILES | O[C@H]1[C@H](N2C=NC3=C2N=C(N)NC3=O)O[C@H](COP(OP(NP([O-])([O-])=O)([O-])=O)([O-])=O)[C@H]1O.[Li+].[Li+].[Li+].[Li+] | ||
| Formula | C10H13N6O13P3 • 4Li | M.Wt | 545.9 |
| Solubility | 10mg/mL in PBS, pH7.2 | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8318 mL | 9.1592 mL | 18.3184 mL |
| 5 mM | 0.3664 mL | 1.8318 mL | 3.6637 mL |
| 10 mM | 0.1832 mL | 0.9159 mL | 1.8318 mL |
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- Purity: >95.00%
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