Hydroxystilbamidine (Synonyms: Oxistilbamidinum; Bis(8-hydroxyquinolinium) sulphate; 2-Hydroxy-4,4'-diguanylstilbene) |
| Catalog No.GC26901 |
Hydroxystilbamidine ((E)-hydroxystilbamidine), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 495-99-8
Sample solution is provided at 25 µL, 10mM.
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Hydroxystilbamidine ((E)-hydroxystilbamidine), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.
Instructions
I. Retrograde neural tracing
1. Material preparation:
Hydroxystilbamidine (usually prepared as a 2% solution).
Solvent: physiological saline, distilled water, or buffer (such as PBS) according to experimental requirements.
Auxiliary materials: syringe or microinjection device.
2. Steps:
(1) Preparation of working solution: prepare a 2% Hydroxystilbamidine solution, which can be sterile filtered.
Note: It is recommended to operate in the dark to prevent fluorescence signal attenuation.
(2) Injection of dye: Inject the dye into the target nerve or tissue area.
Note: Use a microinjector to reduce damage to surrounding tissues.
(3) Retrograde transport: After the injection is completed, wait 24-72 hours to allow the dye to be retrogradely transported to the cell body through the nerve endings.
(4) Tissue sampling: After the animal is killed, remove the tissue of interest and fix it (usually with 4% PFA). After sectioning, observe under a fluorescence microscope.
II. Histochemical staining
1. Material preparation:
(1) Hydroxystilbamidine solution (recommended 1-10 μM).
(2) Cell or tissue sample.
(3) DAPI or other fluorescent contrast dye (optional).
2. Steps:
(1) Fixation and permeabilization:
(2) Fix cells or tissue with 4% PFA.
(3) Permeabilize cell membrane with 0.1-0.5% Triton X-100.
3. Staining:
(1) Immerse the sample in Hydroxystilbamidine solution and incubate for 10-30 minutes in the dark.
(2) Wash 2-3 times to remove unbound dye.
(3) Microscope observation: Use a suitable fluorescence microscope, usually observe fluorescence at 365 nm (UV excitation).
Notes:
(1) Storage in dark place: Hydroxystilbamidine is susceptible to light degradation. The solution should be stored in dark place. It is recommended to store it at 4℃ for short term and at -20℃ for long term.
(2) Operation safety: Avoid direct contact with skin or inhalation. Protective equipment should be worn.
(3) Experimental optimization: Adjust the concentration and incubation time according to the sample type and experimental objectives.
| Cas No. | 495-99-8 | SDF | |
| Synonyms | Oxistilbamidinum; Bis(8-hydroxyquinolinium) sulphate; 2-Hydroxy-4,4'-diguanylstilbene | ||
| Canonical SMILES | C(=CC1=CC=C(C(=N)N)C=C1)C2=C(O)C=C(C(=N)N)C=C2 | ||
| Formula | C16H16N4O | M.Wt | 280.32 |
| Solubility | DMSO: Insoluble | Storage | Store at 2-8°C,protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.5674 mL | 17.8368 mL | 35.6735 mL |
| 5 mM | 0.7135 mL | 3.5674 mL | 7.1347 mL |
| 10 mM | 0.3567 mL | 1.7837 mL | 3.5674 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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