Home>>Signaling Pathways>> Microbiology & Virology>> Fungal>>Hygromycin B

Hygromycin B

Catalog No.GC15496

Hygromycin B Chemical Structure

A selective antibiotic

Size Price Stock Qty
10mM (in 1mL Water)
In stock
In stock
In stock

Customer Reviews

Based on customer reviews.

Tel: (626) 353-8530 Email: sales@glpbio.com

Sample solution is provided at 25 µL, 10mM.

Quality Control

Quality Control & SDS

View current batch:


Animal experiment:

Hygromycin B is dissolved in sterile water. The mice C57BL/6J-TgN(pPWL512hyg)1Ems carrying hygR are treated with a single dose of hygromycin B i.p. at doses that starts at 2.7 mg/kg and increases by 50% for each consecutive dose. Control wild-type C57BL/6J mice are treated with the same volume of sterile saline. Total volume injected is 0.5 mL. The health status and body weights of animals are monitored daily for 10 consecutive days[4].


[1]. Pardo JM, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.
[2]. González A, et al. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978 Dec 21;521(2):459-69.
[3]. Pfister P, et al. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003 May;47(5):1496-502.


Hygromycin B is a selective antibiotic.

Antibiotics are a type of antimicrobial used in the treatment and prevention of bacterial infection.

Hygromycin B is an antibiotic that is effective on most bacteria, fungi and higher eukaryotes. Hygromycin B has a single binding site within the 30S subunit, which is in consistent with its monophasic effect. The binding site is located in the major groove of the helix with a highly sequence-specific way. Hygromycin B inhibits protein synthesis by inhibiting the translocation step of elongation and causing mistranslation of mRNA in the 70S ribosome [1]. Hygromycin B selectively inhibited activity of the ribosomal ATPase (RbbA) on 70S ribosomes and releases RbbA from 70S ribosomes at physiological concentrations. RbbA enhances the reactivity of A889 and G890 of the 16S rRNA switch helix region. Also, Hygromycin B protects G1494 and A1408 in the decoding region.

[1].  Brodersen DE, Clemons WM Jr, Carter AP, et al. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell, 2000, 103(7): 1143-1154.
[2].  Ganoza MC, Kiel MC. A ribosomal ATPase is a target for hygromycin B inhibition on Escherichia coli ribosomes. Antimicrob Agents Chemother, 2001, 45(10): 2813-2819.

Chemical Properties

Cas No. 31282-04-9 SDF
Synonyms N/A
Chemical Name (3'R,3aS,4S,4'R,5'R,6R,6'R,7S,7aS)-4-[(1R,2S,3R,5S,6R)-3-amino-2,6-dihydroxy-5-(methylamino)cyclohexyl]oxy-6'-[(1S)-1-amino-2-hydroxyethyl]-6-(hydroxymethyl)spiro[4,6,7,7a-tetrahydro-3aH-[1,3]dioxolo[4,5-c]pyran-2,2'-oxane]-3',4',5',7-tetrol
Formula C20H37N3O13 M.Wt 527.5
Solubility ≥ 26.375mg/mL in H2O Storage N/A
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

  • Molarity Calculator

  • Dilution Calculator

**When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / CoA (available online).



Review for Hygromycin B

Average Rating: 5 ★★★★★ (Based on Reviews and 38 reference(s) in Google Scholar.)

5 Star
4 Star
3 Star
2 Star
1 Star
Review for Hygromycin B

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.