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HZ-1157

Catalog No.GC13312

HCV NS3/4A protease inhibitor

Products are for research use only. Not for human use. We do not sell to patients.

HZ-1157 Chemical Structure

Cas No.: 1009734-33-1

Size Price Stock Qty
5mg
$72.00
In stock
25mg
$225.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

HZ-1157 is a potent hepatitis C virus (HCV) inhibitor toward HCV NS3/4A protease with IC50 value of 1.0 μmol/L [1].

The NS3 protease of HCV is a prime target for the development of anti-HCV agents because it cleaves the viral polyprotein and liberates NS3, NS4A, NS4B, NS5A, and NS5B, allowing them to function normally in viral RNA replication, and it deactivates many host proteins involved in innate immunity to foster a favorable cellular environment for HCV replication. The NS3 protease is most active when complexed with its cofactor NS4A [1].

In vitro: Huh7.5.1 cell line stably transfected with HCV NS3/4A protease reporter was established to investigate the anti-HCV mechanism of HZ-1157 (0.001~1 μmol/L). The results showed that HZ-1157 exhibited inhibitory effect on the HCV NS3/4A protease with IC50 value of 1.0 μmol/L. HZ-1157 was further tested against an infectious HCV virus (J399EM) which can infect and replicate in Huh7.5.1 cells in vitro. The results showed that HZ-1157 effectively inhibited J399EM virus replication in Huh7.5.1 cells with IC50 value of 0.82 μmol/L [1].

Reference:
[1].  Yu Y, Jing J, Tong X, et al. Discovering novel anti-HCV compounds with inhibitory activities toward HCV NS3/4A protease[J]. Acta Pharmacologica Sinica, 2014, 35(8): 1074.

Chemical Properties

Cas No. 1009734-33-1 SDF
Chemical Name 5-(tert-butoxy)quinazoline-2,4-diamine
Canonical SMILES NC1=NC(N)=NC2=CC=CC(OC(C)(C)C)=C21
Formula C12H16N4O M.Wt 232.28
Solubility ≥ 11.6mg/mL in DMSO Storage Store at -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 4.3051 mL 21.5257 mL 43.0515 mL
5 mM 0.861 mL 4.3051 mL 8.6103 mL
10 mM 0.4305 mL 2.1526 mL 4.3051 mL
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.

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Average Rating: 5 ★★★★★ (Based on Reviews and 39 reference(s) in Google Scholar.)

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