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Janelia Fluor 549, free acid (Synonyms: JF549 TFA)

Catalog No.GC50479

Janelia Fluor 549, free acid (JF549 TFA) is a fluorescent dye with the absorption maximum (Λab (max)) of 549 nm and emission maximum (Λem (max)) of 571 nm.

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Janelia Fluor 549, free acid Chemical Structure

Cas No.: 2245946-45-4

Size Price Stock Qty
5 mg
$594.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Description Chemical Properties Product Documents Related Products

Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.

Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668

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Average Rating: 5 ★★★★★ (Based on Reviews and 20 reference(s) in Google Scholar.)

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