Janelia Fluor 549, free acid (Synonyms: JF549 TFA) |
Catalog No.GC50479 |
Janelia Fluor 549, free acid (JF549 TFA) is a fluorescent dye with the absorption maximum (Λab (max)) of 549 nm and emission maximum (Λem (max)) of 571 nm.
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2245946-45-4
Sample solution is provided at 25 µL, 10mM.
Yellow fluorescent dye; supplied as a free acid. Used in protocol (2017 Grimm et al - see references below) for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands, for use in live cell imaging experiments. Also suitable for flow cytometry. Excitation maximum = 549 nm; emission maximum = 571 nm; Quantum yield = 0.88; Extinction coefficient = 101,000 M-1cm-1.
Grimm et al (2015) A general method to improve fluorophores for live-cell and single-molecule microscopy. Nat. Methods 12 244 PMID:25599551 |Grimm et al (2017) Synthesis of Janelia Fluor HaloTag and SNAP-Tag Ligands and Their Use in Cellular Imaging Experiments. Methods Mol.Biol. 1663 179 PMID:28924668
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