(±)5(6)-DiHET (Synonyms: (±)5,6-DiHETrE) |
カタログ番号GC40270 |
5(6)-DiHETは、エポキシドヒドロラーゼによって生合成された対向異性体形態の完全なラセミ体です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 213382-49-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
5(6)-DiHETは、エポキシドヒドロラーゼによって生合成された対映異性体形態の完全なラセミ体です。[1] 5(6)-EETからの変換により、5(6)-EETを定量するために使用できます。[2] 5(6)-DiHETは、ラット小冠動脈平滑筋細胞内の大型伝導カルシウム活性化カリウム(BK)チャネルを活性化します。[3] 羊精嚢COXの基質としても機能し、in vitroでは5,6-ジヒドロキシプロスタグランジンE1およびF1α代謝物を生成します。[4] また、高脂肪食誘発性ラットモデルの血漿中で5(6)-DiHETレベルが低下することが報告されています。[5]
参考文献:
[1]. Oliw, E.H., Guengerich, F.P., and Oates, J.A. Oxygenation of arachidonic acid by hepatic monooxygenases. Isolation and metabolism of four epoxide intermediates. J. Biol. Chem. 257(7), 3771-3781 (1982).
[2]. Rashid, M., Manivet, P., Nishio, H., et al. Identification of the binding sites and selectivity of sarpogrelate, a novel 5-HT2 antagonist, to human 5-HT2A, 5-HT2B and 5-HT2C receptor subtypes by molecular modeling. Life Sci. 73(2), 193-207 (2003).
[3]. Lu,T、Katakam,P.V.G、VanRollins,M、et al.Dihydroxyeicosatrienoic acids are potent activators of Ca^2+-activated K^+ channels in isolated rat coronary arterial myocytes.J.Physiol.534(Pt3)、651-667(2001)。
[4].Oliw,E.H.Biosynthesisof5,6-dihydroxyprostaglandinE1andF1αfrom5,6-dihydroxyeicosatrienoicacidbyramseminalvesicles.Biochim.Biophys.Acta。795(2)、384-391(1984)。
[5]。Miao,H.Zhao,Y.-H.Vaziri,N.D.et al.Lipidomicsbiomarkersofdiet-inducedhyperlipidemiaanditstreatmentwithPoriacocos.J.Agric.FoodChem。64(4)、969-979(2016)。
Cas No. | 213382-49-1 | SDF | |
同義語 | (±)5,6-DiHETrE | ||
Chemical Name | (±)5,6-dihydroxy-8Z,11Z,14Z-eicosatrienoic acid | ||
Canonical SMILES | CCCCC/C=C\C/C=C\C/C=C\C[C@@H](O)[C@@H](O)CCCC(O)=O | ||
Formula | C20H34O4 | M.Wt | 338.5 |
溶解度 | 50mg/mL in DMSO, 50mg/mL in DMF | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.9542 mL | 14.771 mL | 29.5421 mL |
5 mM | 0.5908 mL | 2.9542 mL | 5.9084 mL |
10 mM | 0.2954 mL | 1.4771 mL | 2.9542 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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