2′-C-β-Methylguanosine |
| カタログ番号GC49823 |
BMS-986094の活性ヌクレオシド代謝物
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 374750-30-8
Sample solution is provided at 25 µL, 10mM.
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2’-C-β-Methylguanosine is an active nucleoside metabolite of the antiviral prodrug BMS-986094.1 It inhibits replication in genotype 1b hepatitis C virus (HCV) replicon cells (EC50s = 5.2 and 3.2 µM in ELISA and luciferase assays, respectively).2 2’-C-β-Methylguanosine also inhibits replication of the Zika virus clinical isolate ZIKVPE243 in infected human brain microvascular endothelial cells (HBMECs), SH-SY5Y cells, and Vero cells (EC50s = 0.88, 2.09, and 1.25 µM, respectively) but is toxic to mice infected with ZIKVPE243 at postnatal day 2 when administered at a dose of 25 mg/kg per day.3
1.Liu, A., Lute, J., Gu, H., et al.Challenges and solutions in the bioanalysis of BMS-986094 and its metabolites including a highly polar, active nucleoside triphosphate in plasma and tissues using LC-MS/MSJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.100029-40(2015) 2.Sizun, G., Pierra, C., Peyronnet, J., et al.Design, synthesis and antiviral evaluation of 2’-C-methyl branched guanosine pronucleotides: The discovery of IDX184, a potent liver-targeted HCV polymerase inhibitorFuture Med. Chem.7(13)1675-1700(2015) 3.Matos de Souza, M.R., Cunha, M.S., Okon, A., et al.In vitro and in vivo characterization of the anti-Zika virus activity of ProTides of 2’-C-β-methylguanosineACS Infect. Dis.6(7)1650-1658(2020)
| Cas No. | 374750-30-8 | SDF | |
| Canonical SMILES | C[C@@]1([C@](O[C@@H]([C@H]1O)CO)([H])N2C3=C(C(N=C(N3)N)=O)N=C2)O | ||
| Formula | C11H15N5O5 | M.Wt | 297.3 |
| 溶解度 | DMF: 2 mg/ml,DMSO: 1 mg/ml,PBS (pH 7.2): 1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.3636 mL | 16.818 mL | 33.6361 mL |
| 5 mM | 0.6727 mL | 3.3636 mL | 6.7272 mL |
| 10 mM | 0.3364 mL | 1.6818 mL | 3.3636 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
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