22-NBD Cholesterol |
| カタログ番号GC46527 |
蛍光コレステロールアナログ
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 78949-95-8
Sample solution is provided at 25 µL, 10mM.
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22-NBD cholesterol is a fluorescent analog of cholesterol that contains a fluorescent nitrobenzoxadiazole (NBD) group.1 It has been used in a variety of in vitro and in vivo applications, including analysis of steroid uptake and esterification, intracellular localization and targeting, and metabolism in mammalian and bacterial cells, and intestinal absorption of cholesterol in hamsters.2,3,4 22-NBD cholesterol displays excitation/emission maxima of 472/540 nm, respectively, when incorporated into 1,2-dimyristoyl-sn-glycero-3-PC vesicles.5 The emission maximum of 22-NBD cholesterol is solvent-dependent and increases as solvent polarity increases.1
1.Craig, I.F., Via, D.P., Mantulin, W.W., et al.Low density lipoproteins reconstituted with steroids containing the nitrobenzoxadiazole fluorophoreJ. Lipid Res.22(4)687-696(1981) 2.Faletrov, Y., Brzostek, A., Plocinska, R., et al.Uptake and metabolism of fluorescent steroids by mycobacterial cellsSteroids11729-37(2017) 3.Frolov, A., Petrescu, A., Atshaves, B.P., et al.High density lipoprotein-mediated cholesterol uptake and targeting to lipid droplets in intact L-cell fibroblasts. A single- and multiphoton fluorescence approachJ. Biol. Chem.275(17)12769-12780(2000) 4.Sparrow, C.P., Patel, S., Baffic, J., et al.A fluorescent cholesterol analog traces cholesterol absorption in hamsters and is esterified in vivo and in vitroJ. Lipid Res.40(10)1747-1757(1999) 5.Ostašov, P., SÝkora, J., BrejchovÁ, J., et al.FLIM studies of 22- and 25-NBD-cholesterol in living HEK293 cells: Plasma membrane change induced by cholesterol depletionChem. Phys. Lipids167-16862-69(2013)
| Cas No. | 78949-95-8 | SDF | |
| Canonical SMILES | C[C@H](CNC1=CC=C([N+]([O-])=O)C2=NON=C12)[C@@]3([H])CC[C@@]4([H])[C@]5([H])CC=C6C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@@]43C | ||
| Formula | C28H38N4O4 | M.Wt | 494.6 |
| 溶解度 | 1 mg/ml in Methanol | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.0218 mL | 10.1092 mL | 20.2184 mL |
| 5 mM | 0.4044 mL | 2.0218 mL | 4.0437 mL |
| 10 mM | 0.2022 mL | 1.0109 mL | 2.0218 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >90.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 40 reference(s) in Google Scholar.)
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