22(S)-hydroxy Cholesterol (Synonyms: 22β-hydroxy Cholesterol) |
カタログ番号GC18270 |
合成オキシステロールおよびLXR調節剤
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Cas No.: 22348-64-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
22(S)-ヒドロキシコレステロールは、合成オキシステロールであり、肝臓X受容体(LXR)の調節因子です。[1] LXRアゴニストGW 3965によって誘導される単球化学足場タンパク質1(MCP-1)発現を原発性肝細胞で防止し、人間筋管内のLXR標的遺伝子CD36、ACSL1、およびSCD-1のmRNA発現をダウンレギュレーションします。濃度が10μMの場合、ラベル付けされたパルミチン酸と酢酸からトリアシルグリセロールおよびジアシルグリセロールの合成を人間筋芽細胞で50%減少させます。 22(S)-ヒドロキシコレステロールはまた、RXRαとLXRαで転染したCOS-1細胞中のプロモーター領域にあるLXR応答要素を介して脂肪酸合成酵素(FAS)報告者活性を低下させます。そしてマウスの慢性エタノール消費モデルではMCP-1およびCCR2の発現を低下させます。[1] [2] 22(S)-ヒドロキシコレステロールの食事補給(30 mg / kg /日)は、通常の飼料または高脂肪食で与えられた場合にも、体重増加が少なく、肝臓トリアシルグリセロールレベルが低くなり、高脂肪食から生じる血漿トリアシルグリセロールレベルの上昇を防止します。[3]
参考文献:
[1]. Kase, E.T., Andersen, B., Nebb, H.I.、他。22-ヒドロキシコレステロールは、人間の筋肉細胞においてT0901317と異なる脂質代謝を調節する。Biochim. Biophys. Acta. 1761(12), 1515-1522 (2006)。
[2]. Na, T.-Y., Han, Y.-H., Ka, N.-L.、他。22-S-ヒドロキシコレステロールは、LXRαによって仲介されるMCP-1の自己/副作用活性化を阻止することでエタノール誘発性肝障害から保護する。J. Pathol. 235(5), 710-720 (2015)。
[3]. Kase, E.T., Nikolić, N., Pettersen Hessvik, N.、他。ラットへの22-S-ヒドロキシコレステロールの食事補給は体重増加と肝臓三酸グリセリドの蓄積を減らす。Lipids 47(5), 483-493 (2012)。
Cas No. | 22348-64-7 | SDF | |
同義語 | 22β-hydroxy Cholesterol | ||
Chemical Name | cholest-5-ene-3β,22S-diol | ||
Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=CC[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@@]4([C@@H]([C@@H](O)CCC(C)C)C)[H] | ||
Formula | C27H46O2 | M.Wt | 402.7 |
溶解度 | 20mg/mL in Ethanol, 0.1mg/mL in DMSO, 2mg/mL in DMF, Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml | Storage | Store at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.4832 mL | 12.4162 mL | 24.8324 mL |
5 mM | 0.4966 mL | 2.4832 mL | 4.9665 mL |
10 mM | 0.2483 mL | 1.2416 mL | 2.4832 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
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