306-O12B |
| カタログ番号GC52149 |
イオン化可能な陽イオン性リピドイド
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2566523-06-4
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
306-O12B is an ionizable cationic lipid used to generate lipid nanoparticles (LNPs). It can be used for liver-specific CRISPR-Cas9 gene editing[1].
306-O12B LNP specifically delivered Cas9 mRNA and sgAngptl3 to liver hepatocytes of wild-type C57BL/6 mice, resulting in a median editing rate of 38.5% and a corresponding 65.2% reduction of serum ANGPTL3 protein. Delivery using 306-O12B LNP was more efficient than delivery with MC-3 LNP, a gold standard LNP[1].
Reference：
[1]: Qiu M, Glass Z,et,al. Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):e2020401118. doi: 10.1073/pnas.2020401118. PMID: 33649229; PMCID: PMC7958351.
Protocol for in vivo genome editing using 306-O12B LNP [1]:
1.Synthesis of 306-O12B LNPs:
LNPs were prepared using a NanoAssemblr microfluidic system.
Lipidoids, cholesterol, phospholipids (DOPC), and methoxypolyethylene glycol (DMG-PEG2000) were dissolved in 100% ethanol at molar ratios of 50/38.5/10/1.5 at a final lipidoids concentration of 10 mg/mL.
The lipid solution was then mixed with an acidic sodium acetate buffer (25 mM, pH 5.2) containing mRNA by using the NanoAssemblr microfluidic system. The weight ratio of 306-O12B/mRNA was 7.5:1.
The resulting LNP was dialyzed against phosphate-buffered saline (PBS) (pH 7.4, 10 mM) overnight at 4 °C.
2.In vivo genome editing (eg：Angptl3）:
Female wild-type C57BL/6 mice, aged 6 to 8 wk, were intravenously dosed with Cas9 mRNA and ANGPTL3-targeted sgRNA coloaded 306-O12B LNP at a dose of 1.0, 2.0, and 3.0 mg/kg in total RNA.
| Cas No. | 2566523-06-4 | SDF | |
| Canonical SMILES | O=C(OCCSSCCCCCCCC)CCN(CCCN(C)CCCN(CCC(OCCSSCCCCCCCC)=O)CCC(OCCSSCCCCCCCC)=O)CCC(OCCSSCCCCCCCC)=O | ||
| Formula | C59H115N3O8S8 | M.Wt | 1251.1 |
| 溶解度 | Ethanol: 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7993 mL | 3.9965 mL | 7.993 mL |
| 5 mM | 0.1599 mL | 0.7993 mL | 1.5986 mL |
| 10 mM | 0.0799 mL | 0.3996 mL | 0.7993 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *