306-O12B |
| カタログ番号GC52149 |
306-O12bは、脂質ナノ粒子（LNP）を生成するために使用されるイオン化可能なカチオン性資質である。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 2566523-06-4
Sample solution is provided at 25 µL, 10mM.
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306-O12bは、脂質ナノ粒子（LNP）を生成するために使用されるイオン化可能なカチオン性資質である。肝臓固有のCRISPR-CAS9遺伝子編集に使える[1]。
306-O12B LNPはCas9 mRNAとSgangptl3を野生型C57BL/6鼠の肝細胞に特異的に送達し、38.5％の編集率の中央値と血清Angptl3タンパク質の対応する65.2％減少をもたらす。306-O12B LNPを使う送達はゴールドスタンダードLNPであるMC-3 LNPを使って、配達よりも効率だ[1]。
Reference：
[1]: Qiu M, Glass Z,et,al. Lipid nanoparticle-mediated codelivery of Cas9 mRNA and single-guide RNA achieves liver-specific in vivo genome editing of Angptl3. Proc Natl Acad Sci U S A. 2021 Mar 9;118(10):e2020401118. doi: 10.1073/pnas.2020401118. PMID: 33649229; PMCID: PMC7958351.
306-O12B LNP を使う体内ゲノム編集のプロトコル[1]：
1. 306-O12B LNPの合成：
ナノAssemblrマイクロ流体システムでLNPを調製される。
脂質、コレステロール、リン脂質（DOPC）、methoxypolyethylene glycol (DMG-PEG2000)は最終脂質濃度10 mg/mlで50/38.5/10/1.5のモル比で100％エタノールに溶解する。
次に脂質溶液をナノAssemblrマイクロ流体システムでmRNAを含んでいる酸性酢酸ナトリウム緩衝液（25 mM，pH 5.2）と混合する。306-O12b/mRNA重量比は7.5：１である
もらったLNPは4°Cで一晩、リン酸緩衝生理食塩水（PBS）（pH 7.4，10 mm）に対して透析する。
2.在体内基因组编辑中（例如：angptl3）：
生体内の編集（例：angptl3）：
6～8週齢のメスの野生型C57BL/6鼠にCas9 mRNAが1.0、2.0，2.0 mg/kgの用量で総mRNAが1.0、2.0 mg/kgの用量で静脈注射する。
| Cas No. | 2566523-06-4 | SDF | |
| Canonical SMILES | O=C(OCCSSCCCCCCCC)CCN(CCCN(C)CCCN(CCC(OCCSSCCCCCCCC)=O)CCC(OCCSSCCCCCCCC)=O)CCC(OCCSSCCCCCCCC)=O | ||
| Formula | C59H115N3O8S8 | M.Wt | 1251.1 |
| 溶解度 | Ethanol: 10 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 0.7993 mL | 3.9965 mL | 7.993 mL |
| 5 mM | 0.1599 mL | 0.7993 mL | 1.5986 mL |
| 10 mM | 0.0799 mL | 0.3996 mL | 0.7993 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
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