3X FLAG Peptide (Synonyms: H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH) |
カタログ番号GP10149 |
合成ペプチドタグ
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 402750-12-3
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Protocol for Protein expression and purification by 3×FLAG peptide [1]: |
This protocol only provides a guideline, and should be modified according to your specific needs. |
Protocol for Co-IP and co-IP–MS analyses using 3×FLAG peptide [2]: |
This protocol only provides a guideline, and should be modified according to your specific needs. |
References: [1]. Gao XK, Rao XS, et al. Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discov. 2022 Jun 28;8(1):60. doi: 10.1038/s41421-022-00426-x. PMID: 35764611; PMCID: PMC9240053. [2]. Cong M, Wang Y, et al. MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination. Nat Cancer. 2020 Feb;1(2):222-234. doi: 10.1038/s43018-019-0021-y. Epub 2020 Jan 20. PMID: 35122005. |
FLAGタグシステムは、興味のあるタンパク質に融合された短い親水性8アミノ酸ペプチドを利用します。FLAGペプチドは抗体M1に結合します。結合がカルシウム依存的2または非依存的3であるかどうかは議論があります。このシステムの欠点は、モノクローナル抗体精製マトリックスが他のものほど安定していないことです。一般的に、小さなタグは特異的なモノクローナル抗体で検出することができます。
FLAGタグの検出を改善するために、3x FLAGシステムが開発されました。この三連続FLAGエピトープは親水性で22アミノ酸長であり、発現した融合タンパク質を10 fmolまで検出することができます。Pyrococcus furiosusのFLAG付きマルトデキストリン結合タンパク質が結晶化され4、その結晶品質は未修飾タンパク質のそれと非常に似ていました。
最後に、enterokinase処理により5つのC末端アミノ酸に特異的なFLAGタグを除去することができます。
参考文献:
1. Hopp TP、Prickett KS、Price VL、Libby RT、March CJ、Ceretti DP、Urdal DL、Conlon PJ(1988)「再生タンパク質の同定と精製に有用な短いポリペプチドマーカー配列」Bio/Technology 6:1204-1210。
2. Hopp TP、Gallis B、Prikett KS(1996)「カルシウム依存性モノクローナル抗体の金属結合特性」Mol Immunol 33:601-608。
3. Einhauer A, Jungbauer A (2000) 「FLAGペプチドに対するモノクローナル抗体M1の運動学的および熱力学的特性」20th International symposium on the separation of proteins, peptides, and polynucleotides (ISPPP). Lublijana, Slovenia, November 5-8, 2000.
4. Bucher MH, Evdokimov AG, Waugh DS (2002) 「Pyrococcus furiosus maltodextrin-binding proteinの結晶化に対する短い親和タグの異なる効果」Biol Cryst 58:392-397。
5. Maroux S, Baratti J, Desnuelle P (1971) 「豚腸エンテロキナーゼの精製と特異性」J Biol Chem 246:5031-5039。
Cas No. | 402750-12-3 | SDF | |
同義語 | H-Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH | ||
Chemical Name | 3X FLAG Peptide | ||
Canonical SMILES | CCC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(CC1=CC=C(C=C1)O)C(=O)NC(CCCCN)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC(CCCCN)C(=O)O)NC(=O)C(CC(=O)O)NC(=O)C(CC2=CNC=N2)NC(=O)C(CC(=O)O)NC(=O)C(CCCCN)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C(CC(=O)O)NC( | ||
Formula | C120H169N31O49S | M.Wt | 2861.87 |
溶解度 | ≥143.1mg/mL in DMSO, <14.35mg/mL in EtOH, ≥143.4mg/mL in Water with gentle warming | Storage | Desiccate at -20°C |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.3494 mL | 1.7471 mL | 3.4942 mL |
5 mM | 0.0699 mL | 0.3494 mL | 0.6988 mL |
10 mM | 0.0349 mL | 0.1747 mL | 0.3494 mL |
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
(Based on Reviews and 29 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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