5β,6β-epoxy Cholestanol (Synonyms: NSC 148940) |
| カタログ番号GC40418 |
5β,6β-エポキシコレスタノールは、コレステロールの酸化代謝物であり、ラジカルおよび非ラジカル酸化によって5,6-二重結合でコレステロールから形成されます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 4025-59-6
Sample solution is provided at 25 µL, 10mM.
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5β,6β-epoxy Cholestanol is an oxidative metabolite of cholesterol that is formed via radical and non-radical oxidation of cholesterol at the 5,6-double bond.[1] [2] It induces release of lactate dehydrogenase (LDH) and apoptosis in macrophage-differentiated U937 cells.[3] 5β,6β-epoxy Cholestanol has been found in human fatty streaks and advanced atherosclerotic lesions but is not present in normal aortic tissue.[4]
Reference:
[1]. Pulfer, M.K., and Murphy, R.C. Formation of biologically active oxysterols during ozonolysis of cholesterol present in lung surfactant. The Journal of Biological Chemisty 279(25), 26331-26338 (2004).
[2]. Aringer, L., and Eneroth, P. Formation and metabolism in vitro of 5,6-epoxides of cholesterol and β-sitosterol. J. Lipid Res. 15(4), 389-398 (1974).
[3]. Lordan, S., O'Brien, N.M., and Mackrill, J.J. The role of calcium in apoptosis induced by 7β-hydroxycholesterol and cholesterol-5β,6β-epoxide. J. Biochem. Mol. Toxicol. 23(5), 324-332 (2009).
[4]. Garcia-Cruset, S., Carpenter, K.L., Guardiola, F., et al. Oxysterol profiles of normal human arteries, fatty streaks and advanced lesions. Free Radic. Res. 35(1), 31-41 (2001).
| Cas No. | 4025-59-6 | SDF | |
| 同義語 | NSC 148940 | ||
| Chemical Name | 5β,6β-epoxy-cholestan-3β-ol | ||
| Canonical SMILES | C[C@H](CCCC(C)C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])C[C@@H]4[C@]5(O4)C[C@@H](O)CC[C@]5(C)[C@@]3([H])CC[C@@]21C | ||
| Formula | C27H46O2 | M.Wt | 402.7 |
| 溶解度 | ≤20mg/ml in Ethanol; 0.1mg/ml in DMSO; 2mg/ml in DMF; Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4832 mL | 12.4162 mL | 24.8324 mL |
| 5 mM | 0.4966 mL | 2.4832 mL | 4.9665 mL |
| 10 mM | 0.2483 mL | 1.2416 mL | 2.4832 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 34 reference(s) in Google Scholar.)
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