5-Bromo-4-chloro-3-indolyl-β-D-Glucuronide (cyclohexylammonium salt hydrate) (Synonyms: BCIG, X-GLUC) |
| カタログ番号GC48435 |
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
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5-Bromo-4-chloro-3-indolyl-β-D-glucuronide (X-GLUC) is a chromogenic substrate for β-glucuronidase.1,2 Upon cleavage by β-glucuronidase, an indigo pigment is released that can be used as a marker of β-glucuronidase activity. X-GLUC has been used for the detection of E. coli contamination in foodstuffs.
1.Frampton, E.W., Restaino, L., and Blaszko, N.Evaluation of the β-glucuronidase substrate 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-GLUC) in a 24-hour direct plating method for Escherichia coliJ. Food Prot.51(5)402-404(1988) 2.Restaino, L., Frampton, E.W., and Lyon, R.H.Use of the chromogenic substrate 5-bromo-4-chloro-3-indolyl-B-D-glucuronide (X-GLUC) for enumerating Escherichia coli in 24 H from ground beefJ. Food Prot.53(6)508-510(1990)
| Cas No. | SDF | ||
| 同義語 | BCIG, X-GLUC | ||
| Canonical SMILES | NC1CCCCC1.BrC2=CC=C3C(C(O[C@@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)C(O)=O)=CN3)=C2Cl.O | ||
| Formula | C14H13BrClNO7•C6H13N [H2O] | M.Wt | 539.8 |
| 溶解度 | DMF: 10 mg/ml,DMSO: 10 mg/ml,Ethanol: 0.50 mg/ml,PBS (pH 7.2): 1 mg/ml | Storage | -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.8525 mL | 9.2627 mL | 18.5254 mL |
| 5 mM | 0.3705 mL | 1.8525 mL | 3.7051 mL |
| 10 mM | 0.1853 mL | 0.9263 mL | 1.8525 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 34 reference(s) in Google Scholar.)
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