7β,27-dihydroxy Cholesterol (Synonyms: 7β,27-DHC) |
| カタログ番号GC46740 |
オキシステロールであり、RORγおよびRORγtのアゴニスト
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 240129-43-5
Sample solution is provided at 25 µL, 10mM.
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7β,27-dihydroxy Cholesterol is an oxysterol and agonist of retinoic acid receptor-related orphan receptor γ (RORγ) and RORγt. [1] It activates RORγ- or RORγt-dependent signaling with EC50 values of 691 and 1,045 nM, respectively, in reporter assays using HEK293T cells expressing the recombinant human receptors. 7β,27-dihydroxy Cholesterol is selective for RORγ and RORγt over a panel of eight additional nuclear receptors at 30 µM. It increases IL-17A production in Th17-polarized isolated human naÏve CD4+ T cells when used at a concentration of 300 nM. 7β,27-dihydroxy Cholesterol (60 mg/kg) increases IL-17A production in isolated mouse γδ T cells stimulated with 12-myristate 13-acetate and ionomycin .
Reference:
[1].Soroosh, P., Wu, J., Xue, X., et al.Oxysterols are agonist ligands of RORγt and drive Th17 cell differentiationProc. Natl. Acad. Sci. USA111(33)12163-12168(2014)
| Cas No. | 240129-43-5 | SDF | |
| 同義語 | 7β,27-DHC | ||
| Chemical Name | (3β,7β,25R)-cholest-5-ene-3,7,26-triol | ||
| Canonical SMILES | O[C@H](C1)CC[C@@]2(C)C1=C[C@H](O)[C@]3([H])[C@]2([H])CC[C@@]4(C)[C@@]3([H])CC[C@]4([H])[C@H](C)CCC[C@@H](C)CO | ||
| Formula | C27H46O3 | M.Wt | 418.7 |
| 溶解度 | DMF: 2 mg/ml,Ethanol: 20 mg/ml,Ethanol:PBS (pH 7.2) (1:2): 0.30 mg/ml | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.3883 mL | 11.9417 mL | 23.8834 mL |
| 5 mM | 0.4777 mL | 2.3883 mL | 4.7767 mL |
| 10 mM | 0.2388 mL | 1.1942 mL | 2.3883 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 6 reference(s) in Google Scholar.)
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