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β-Nicotinamide mononucleotide (Synonyms: βNMN)

カタログ番号GC16971

β-ニコチンアミドモノヌクレオチドは、ニコチンアミドリン酸フォスホリボシルトランスフェラーゼ(NAMPT)反応の生成物であり、重要なNAD +中間体です。

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β-Nicotinamide mononucleotide 化学構造

Cas No.: 1094-61-7

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100mg
$37.00
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500mg
$71.00
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Sample solution is provided at 25 µL, 10mM.

Product has been cited by 1 publications

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

Hepa1-6 cells

Preparation Method

β-Nicotinamide mononucleotide was added to the cell medium

Reaction Conditions

0.5-1 mMβ-Nicotinamide mononucleotide

Applications

Intracellular NAD + levels were significantly reduced by knocking down or knocking down Nampt or treated with the Nampt inhibitor FK866, whereas NAD + levels were significantly increased by supplementation with NAD + precursors NAM or β-Nicotinamide mononucleotide.

Animal experiment [2]:

Animal models

Nampt+/- female mice

Preparation Method

For GTTs, mice were injected with PBS or β-Nicotinamide mononucleotide (500 mg/kg body weight) and fasted for 14 hrs; dextrose (3 g/kg body weight) was then injected intraperitoneally.

Dosage form

500 mg/kg β-Nicotinamide mononucleotide for 0、15、30、60,120min

Applications

After β-Nicotinamide mononucleotide administration, there was no difference in blood glucose levels in GTTs between Nampt+/- and control female mice. In addition, β-Nicotinamide mononucleotide-treated Nampt+/- and control mice also had similar plasma insulin levels at each time point. β-Nicotinamide mononucleotide administration corrects the defect in GSIS observed in Nampt+/- mice.

References:

[1]: Lv H, Lv G, et,al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Cell Metab. 2021 Jan 5;33(1):110-127.e5. doi: 10.1016/j.cmet.2020.10.021. Epub 2020 Nov 9. PMID: 33171124.
[2]: Revollo JR, KÖrner A, et,al. Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme. Cell Metab. 2007 Nov;6(5):363-75. doi: 10.1016/j.cmet.2007.09.003. PMID: 17983582; PMCID: PMC2098698.

Background

β-ニコチンアミド一リン酸モノヌクレオチドは、ニコチンアミドフォスホリボシルトランスフェラーゼ(NAMPT)反応の生成物であり、重要なNAD +中間体です。 β-ニコチンアミド一リン酸モノヌクレオチドの薬理学的活性には、細胞生化学機能、心臓保護、糖尿病、アルツハイマー病、肥満に関連する合併症が含まれます[1]。

細胞内のNAD+レベルは、Namptをノックダウンしたり、Nampt阻害剤FK866で処理したりすることによって有意に低下しました。一方、NAD+前駆体のNAMまたはβ-ニコチンアミド一リン酸を補充することによって、NAD+レベルが有意に増加しました[3]。HEK293細胞へのβ-ニコチンアミド一リン酸(NAD+前駆体)の投与は、核内での塩基配列複製速度を向上させるために、線維芽細胞中で活性化されます。これは、粒子数が減少している代わりに核内で新しい塩基配列が生成されています[7]。

β-ニコチンアミドモノヌクレオチドの投与後、Nampt+/-と対照群の雌マウスにおいてGTT中の血糖値に差は見られませんでした。さらに、β-ニコチンアミドモノヌクレオチドを投与されたNampt+/-と対照群のマウスは、各時間点で同様の血漿インスリン値を示しました。β-ニコチンアミドモノヌクレオチド投与により、Nampt+/-マウスで観察されたGSISの欠陥が修正されます[2]。 β-ニコチンアミド モ ノ ヌ クレオ チッド は HFD 誘導性 T2D マウス における NAD + 水準 の回復によってグルコース不耐性を改善します。また、SIRT1 の活性化を介して一部酸化ストレス、炎症反応、循環リズム関連遺伝子発現も回復します[4]。臨床的な低用量分割投与法で誘導されたマウス模型では、NAD + 前駆体補充により mtDNA 減少と心機能障害を防止できました[5]。全身動物実験において、β-ニコチンアミドモノヌクレオチドがNAD + 前駆体としてニコチンアミド(Nam)よりも優れているかどうか調べた結果、β-ニコチンアミド モ ノ ヌ クレオ チッド は Nam よりも体内に長く留まることがわかりました[6]

References:
[1]: Poddar SK, Sifat AE, et,al. Nicotinamide Mononucleotide: Exploration of Diverse Therapeutic Applications of a Potential Molecule. Biomolecules. 2019 Jan 21;9(1):34. doi: 10.3390/biom9010034. PMID: 30669679; PMCID: PMC6359187.
[2]: Revollo JR, KÖrner A, et,al. Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme. Cell Metab. 2007 Nov;6(5):363-75. doi: 10.1016/j.cmet.2007.09.003. PMID: 17983582; PMCID: PMC2098698.
[3]: Lv H, Lv G, et,al. NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion. Cell Metab. 2021 Jan 5;33(1):110-127.e5. doi: 10.1016/j.cmet.2020.10.021. Epub 2020 Nov 9. PMID: 33171124.
[4]:Yoshino J, Mills KF, et,al. Nicotinamide mononucleotide, a key NAD(+) intermediate, treats the pathophysiology of diet- and age-induced diabetes in mice. Cell Metab. 2011 Oct 5;14(4):528-36. doi: 10.1016/j.cmet.2011.08.014. PMID: 21982712; PMCID: PMC3204926.
[5]: Li J, Wang PY, et,al. p53 prevents doxorubicin cardiotoxicity independently of its prototypical tumor suppressor activities. Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19626-19634. doi: 10.1073/pnas.1904979116. Epub 2019 Sep 5. PMID: 31488712; PMCID: PMC6765288.
[6]: Kawamura T, Mori N, et,al. β-Nicotinamide Mononucleotide, an Anti-Aging Candidate Compound, Is Retained in the Body for Longer than Nicotinamide in Rats. J Nutr Sci Vitaminol (Tokyo). 2016;62(4):272-276. doi: 10.3177/jnsv.62.272. PMID: 27725413.
[7]:Cros C, Margier M, et,al. Nicotinamide Mononucleotide Administration Triggers Macrophages Reprogramming and Alleviates Inflammation During Sepsis Induced by Experimental Peritonitis. Front Mol Biosci. 2022 Jun 27;9:895028. doi: 10.3389/fmolb.2022.895028. PMID: 35832733; PMCID: PMC9271973.

Chemical Properties

Cas No. 1094-61-7 SDF
同義語 βNMN
Chemical Name ((2R,3S,4R,5R)-3,4-dihydroxy-5-(3-(hydroxy(imino)methyl)pyridin-1-ium-1-yl)tetrahydrofuran-2-yl)methyl hydrogen phosphate
Canonical SMILES N=C(O)C1=C[N+]([C@@]2([H])[C@@](O)([H])[C@@](O)([H])[C@@](O2)([H])COP(O)([O-])=O)=CC=C1
Formula C11H16N2O8P M.Wt 334.22
溶解度 ≥ 33.4mg/mL in Water Storage 4°C, protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 2.992 mL 14.9602 mL 29.9204 mL
5 mM 0.5984 mL 2.992 mL 5.9841 mL
10 mM 0.2992 mL 1.496 mL 2.992 mL
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    Beta-Nicotinamide mononucleotide-GlpBio

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