Betanin |
| カタログ番号GC31409 |
ベタニンには、強力な抗酸化作用と抗炎症作用があり、ペルオキシニトライト（ONOO-）を19.2μMのIC50で阻害することができます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 7659-95-2
Sample solution is provided at 25 µL, 10mM.
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Betanin is a red glycoside extracted from beets. It is a water-soluble nitrogen-containing pigment that can be used as a food colorant[1]. Betanin is unstable and easily degraded under light and high temperature[2]. Betanin has anti-inflammatory and antioxidant activities and also has neuroprotective effects[3].
In vitro, Betanin (10-80µM) treatment of human chronic myeloid leukemia cell line K562 cells for 12-48h dose- and time-dependently inhibited cell viability, induced cell apoptosis, induced the release of cytochrome c from mitochondria to the cytosol, and reduced membrane potential[4]. Betanin (15μM) treatment of THP-1 cells for 24h significantly induced the expression of IL-1β, IL-8, and IL-10 mRNA in cells[5].
In vivo, oral administration of Betanin (100mg/kg) to a neurodegenerative disease model mouse for 2 weeks had a significant anxiolytic effect, prevented hippocampal CA1 degeneration and changes in choline acetyltransferase (ChAT) activity, and improved spatial learning and memory impairment[6]. Oral administration of Betanin (20mg/kg) to a hyperlipidemic diet-induced oxidative stress rat for 20 days reduced liver malondialdehyde content, increased superoxide dismutase, catalase, and glutathione peroxidase activities, and reversed liver tissue damage[7].
References:
[1] Coy-Barrera E. Analysis of betalains (betacyanins and betaxanthins)[M]//Recent advances in natural products analysis. Elsevier, 2020: 593-619.
[2] Azeredo H M C. Betalains: properties, sources, applications, and stability–a review[J]. International journal of food science & technology, 2009, 44(12): 2365-2376.
[3] Thong-Asa W, Puenpha K, Lairaksa T, et al. Neuroprotective effects of betanin in mice with cerebral ischemia-reperfusion injury[J]. Experimental Animals, 2023, 72(3): 336-345.
[4] Sreekanth D, Arunasree M K, Roy K R, et al. Betanin a betacyanin pigment purified from fruits of Opuntia ficus-indica induces apoptosis in human chronic myeloid leukemia Cell line-K562[J]. Phytomedicine, 2007, 14(11): 739-746.
[5] Muramatsu D, Uchiyama H, Higashi H, et al. Effects of heat degradation of betanin in red beetroot (Beta vulgaris L.) on biological activity and antioxidant capacity[J]. Plos one, 2023, 18(5): e0286255.
[6] Thong-Asa W, Prasartsri S, Klomkleaw N, et al. The neuroprotective effect of betanin in trimethyltin-induced neurodegeneration in mice[J]. Metabolic Brain Disease, 2020, 35: 1395-1405.
[7] da Silva D V T, Pereira A D A, Boaventura G T, et al. Short-term betanin intake reduces oxidative stress in wistar rats[J]. Nutrients, 2019, 11(9): 1978.
| Cell experiment [1]: | |
Cell lines | K562 cells |
Preparation Method | Cells were placed in a 96-well plate and incubated with or without Betanin (10, 20, 40, 80µM) for 12, 24, and 48h. Cell viability was determined by MTT assay. |
Reaction Conditions | 10, 20, 40, 80µM; 12, 24, 48h |
Applications | Betanin inhibits cell viability in a dose- and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | ICR mice |
Preparation Method | Forty male ICR mice were randomly divided into four groups: Sham-veh, TMT-veh, TMT-Bet50 and TMT-Bet100. In the TMT groups, neurodegeneration was induced with a one-time intraperitoneal injection of 2.6mg/kg TMT. Betanin-treated groups (Bet) were given oral doses of 50 or 100mg/kg dissolved in normal saline solution. Administrations were started 24h prior to TMT injection and continued for 2 weeks. Anxious behavior and spatial cognition were evaluated, respectively. After behavioral tests, brain oxidative status, hippocampal histology and choline acetyltransferase (ChAT) activity were evaluated. |
Dosage form | 50、100mg/kg for 2 weeks; p.o. |
Applications | Betanin 100mg/kg exhibited significant anxiolytic effect, preventive effect on CA1 degeneration and CA1 ChAT activity alteration as well as improvement of spatial learning and memory deficits. |
References: | |
| Cas No. | 7659-95-2 | SDF | |
| Canonical SMILES | OC([C@@H]1CC(/C=C/N2C3=CC(O)=C(O[C@@H]4O[C@@H]([C@@H](O)[C@H](O)[C@H]4O)CO)C=C3C[C@H]2C(O)=O)=CC(C(O)=O)=N1)=O | ||
| Formula | C24H26N2O13 | M.Wt | 550.47 |
| 溶解度 | Water : 150 mg/mL (272.49 mM) | Storage | Store at -20°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8166 mL | 9.0831 mL | 18.1663 mL |
| 5 mM | 0.3633 mL | 1.8166 mL | 3.6333 mL |
| 10 mM | 0.1817 mL | 0.9083 mL | 1.8166 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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