BODIPY-aminoacetaldehyde diethyl acetal (Synonyms: BAAA-DA) |
カタログ番号GC46939 |
蛍光性ALDH基質BAAAの安定した前駆体
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Cas No.: 247069-93-8
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
BODIPY-アミノアセトアルデヒドジエチルアセタール(BAAA-DA)は、アルデヒド脱水素酵素(ALDH)の細胞浸透性蛍光基質であるBODIPY-アミノアセトアルデヒドの安定前駆体です。12 BODIPY-アミノアセトアルデヒドジエチル アセタールは、酸性条件下でBODIPY- ア ミ ノ ア セ ト ア ル デ ヒ ド ( BAAA )に変換されます。2 BAAAは細胞内でALDHによって変換されて、細胞に保持され、高いALDH活性を有する細胞を識別するために使用できるBODIPY aminoacetate(BAA)になります。 1 BAAはエフラックスポンプP-グリコタンパク質(P-gp)の基質ですが、BAAAとバーパミルなどのP-gp阻害剤を併用することで、BAA排出を抑制できます。2 BAAA-DAは、高いALDH活性を有する人間の造血祖細胞を分離し、ALDHレベルが高い癌幹細胞を流式細胞分析によって選別するために使用されています。 1 3BAAは488 nmで励起され、512 nmの放射最大値が表示されます。4
1. Storms、R.W.、Trujillo、A.P.、Springer、J.B.ら。アルデヒド脱水素酵素活性に基づく原始的な人間造血祖細胞の分離。米国科学アカデミー紀要96(16):9118-9123(1999年) 2. Smith, C.A., Colvin, M., Storms, R.W., et al.BODIPY aminoacetaldehyde diethyl acetal08010501.81-15(2010) 3.Leng, Z., Yang, Z., Li, L., et al.ALDH高癌幹細胞の流式細胞分離と同定の信頼できる方法。Exp.Ther.Med。(2017) 4.Pomper,M.G.、Wang,H.、Minn,I.et al.Red fluorescent aldehyde dehydrogenase (ALDH) substrate(2015)
Cas No. | 247069-93-8 | SDF | |
同義語 | BAAA-DA | ||
Canonical SMILES | [F-][B+3]1([N-]2C(C=C(C(C)=C3)[N]1=C3C)=CC=C2CCC(NCC(OCC)OCC)=O)[F-] | ||
Formula | C20H28BF2N3O3 | M.Wt | 407.3 |
溶解度 | Storage | Store at -20°C,protect from light | |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 2.4552 mL | 12.276 mL | 24.5519 mL |
5 mM | 0.491 mL | 2.4552 mL | 4.9104 mL |
10 mM | 0.2455 mL | 1.2276 mL | 2.4552 mL |
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Average Rating: 5
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