ホーム>>Labeling & Detection>> Fluorescence>>BODIPY-aminoacetaldehyde diethyl acetal

BODIPY-aminoacetaldehyde diethyl acetal (Synonyms: BAAA-DA)

カタログ番号GC46939

蛍光性ALDH基質BAAAの安定した前駆体

Products are for research use only. Not for human use. We do not sell to patients.

BODIPY-aminoacetaldehyde diethyl acetal 化学構造

Cas No.: 247069-93-8

サイズ 価格 在庫数 個数
50 μg
$101.00
在庫あり
100 μg
$192.00
在庫あり
500 μg
$809.00
在庫あり
1 mg
$1,415.00
在庫あり

Tel:(909) 407-4943 Email: sales@glpbio.com

顧客レビュー

カスタマーレビューに基づきます。

  • GlpBio Citations

    GlpBio Citations
  • Bioactive Compounds Premium Provider

    Bioactive Compounds Premium Provider

Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

View current batch:

Background

BODIPY-アミノアセトアルデヒドジエチルアセタール(BAAA-DA)は、アルデヒド脱水素酵素(ALDH)の細胞浸透性蛍光基質であるBODIPY-アミノアセトアルデヒドの安定前駆体です。12 BODIPY-アミノアセトアルデヒドジエチル アセタールは、酸性条件下でBODIPY- ア ミ ノ ア セ ト ア ル デ ヒ ド ( BAAA )に変換されます。2 BAAAは細胞内でALDHによって変換されて、細胞に保持され、高いALDH活性を有する細胞を識別するために使用できるBODIPY aminoacetate(BAA)になります。 1 BAAはエフラックスポンプP-グリコタンパク質(P-gp)の基質ですが、BAAAとバーパミルなどのP-gp阻害剤を併用することで、BAA排出を抑制できます。2 BAAA-DAは、高いALDH活性を有する人間の造血祖細胞を分離し、ALDHレベルが高い癌幹細胞を流式細胞分析によって選別するために使用されています。 1 3BAAは488 nmで励起され、512 nmの放射最大値が表示されます。4

1. Storms、R.W.、Trujillo、A.P.、Springer、J.B.ら。アルデヒド脱水素酵素活性に基づく原始的な人間造血祖細胞の分離。米国科学アカデミー紀要96(16):9118-9123(1999年) 2. Smith, C.A., Colvin, M., Storms, R.W., et al.BODIPY aminoacetaldehyde diethyl acetal08010501.81-15(2010) 3.Leng, Z., Yang, Z., Li, L., et al.ALDH高癌幹細胞の流式細胞分離と同定の信頼できる方法。Exp.Ther.Med。(2017) 4.Pomper,M.G.、Wang,H.、Minn,I.et al.Red fluorescent aldehyde dehydrogenase (ALDH) substrate(2015)

Chemical Properties

Cas No. 247069-93-8 SDF
同義語 BAAA-DA
Canonical SMILES [F-][B+3]1([N-]2C(C=C(C(C)=C3)[N]1=C3C)=CC=C2CCC(NCC(OCC)OCC)=O)[F-]
Formula C20H28BF2N3O3 M.Wt 407.3
溶解度 Storage Store at -20°C,protect from light
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 2.4552 mL 12.276 mL 24.5519 mL
5 mM 0.491 mL 2.4552 mL 4.9104 mL
10 mM 0.2455 mL 1.2276 mL 2.4552 mL
  • モルアリティ計算機

  • 希釈計算機

質量
=
濃度
x
容積
x
MW*
 
 
 
**ストックソリューションを準備する際には、常にバイアルラベルおよび MSDS/CoA(オンラインで利用可能)で掲載された製品のロット固有分子量を使用してください。

計算

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

レビュー

Review for BODIPY-aminoacetaldehyde diethyl acetal

Average Rating: 5 ★★★★★ (Based on Reviews and 34 reference(s) in Google Scholar.)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%
Review for BODIPY-aminoacetaldehyde diethyl acetal

GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.

Required fields are marked with *

You may receive emails regarding this submission. Any emails will include the ability to opt-out of future communications.