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DYKDDDDK Peptide (trifluoroacetate salt) (Synonyms: DYKDDDDK Epitope, DYKDDDDK Octapeptide, DYKDDDDK Tag)

カタログ番号GC48346

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DYKDDDDK Peptide (trifluoroacetate salt) 化学構造

サイズ 価格 在庫数 個数
1mg
$83.00
在庫あり
5mg
$378.00
在庫あり
10mg
$672.00
在庫あり
25mg
$1,470.00
在庫あり

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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Background

DYKDDDDK peptide is a synthetic octapeptide and fusion tag.1,2 It can be added to the N- or C-terminus of proteins or inserted into the protein sequence and later cleaved using enteropeptidase. Due to its hydrophilicity, it has a low potential to interfere with protein folding or activity. DYKDDDDK peptide has commonly been used as an epitope tag on recombinant proteins to facilitate their detection and purification by DYKDDDDK-specific antibodies in vitro and in vivo.1,2,3,4

1.Hopp, T.P., Prickett, K.S., Price, V.L., et al.A short polypeptide marker sequence useful for recombinant protein identification and purificationNat. Biotechnol.61204-1210(1988) 2.Einhauer, A., and Jungbauer, A.The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteinsJ. Biochem. Biophys. Methods49(1-3)455-465(2001) 3.Ichiki, T., Koga, T., Okuno, T., et al.Modulation of leukotriene B4 receptor 1 signaling by receptor for advanced glycation end products (RAGE)FASEB J.30(5)1811-1822(2016) 4.Ikeda, K., Koga, T., Sasaki, F., et al.Generation and characterization of a human-mouse chimeric high-affinity antibody that detects the DYKDDDDK FLAG peptideBiochem. Biophys. Res. Commun.486(4)1077-1082(2017)

Chemical Properties

Cas No. SDF
同義語 DYKDDDDK Epitope, DYKDDDDK Octapeptide, DYKDDDDK Tag
Canonical SMILES OC(C=C1)=CC=C1C[C@H](NC([C@@H](N)CC(O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=OOC(C=C1)=CC=C1C[C@H](NC([C@@H](N)CC(O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O.OC(C(F)(F)F)=O
Formula C41H60N10O20•XCF3COOH M.Wt 1013
溶解度 DMF: 1 mg/ml,DMSO: 10 mg/ml,PBS (pH 7.2): 10 mg/ml Storage -20°C
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

Prepare stock solution
1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9358 mL 9.8717 mL
5 mM 0.1974 mL 0.9872 mL 1.9743 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL
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**ストックソリューションを準備する際には、常にバイアルラベルおよび MSDS/CoA(オンラインで利用可能)で掲載された製品のロット固有分子量を使用してください。

計算

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.

レビュー

Review for DYKDDDDK Peptide (trifluoroacetate salt)

Average Rating: 5 ★★★★★ (Based on Reviews and 32 reference(s) in Google Scholar.)

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