Concanavalin A |
| カタログ番号GC18572 |
コンカナマイシンAは、高い活性と選択的なバキュオールプロトン-ATPase（v-[H+]ATPase）阻害剤である、Streptomyces diastatochromogenesから分離されたマクロライド系抗生物質の一種であるコンカナマイシンに属しています。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 11028-71-0
Sample solution is provided at 25 µL, 10mM.
Concanavalin A belongs to the concanamycins, a family of macrolide antibiotics isolated from Streptomyces diastatochromogenes that are highly active and selective inhibitors of the vacuolar proton-ATPase (v-[H[+]]ATPase). Among them, Concanavalin A is the most selective and potent inhibitor of the V-[H[+]]ATPase.[1]
n vitro study indicated that Concanavalin A was active at 5 nM and completely blocked influenza virus infection at 10 nM in MDCK cells. Results showed that concanamycin A blocked viral replication by inhibiting the v-[H[+]]ATPase, thus preventing acidification of endosomes and release of virions into the cytoplasm. An early event in virus infection is the target of concanamytin A. In addition, the inhibition of the v-[H[+]]ATPase by concanamycin A prevents endosomal acidification, inhibiting virus release from endosomes.[1]
References:
[1].Rosario G. et al. Concanavalin A blocks influenza virus entry into cells under acidic conditions. FEBS Letters 1994 June; 349; 327-330.
| Cell experiment [1]: | |
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Cell lines |
CD8+ CTL |
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Preparation Method |
Cells were cultured in the RPMI 1640 medium supplemented with 10% (v/v) FCS and 5% (v/v) culture supernatant of rat spleen cells. |
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Reaction Conditions |
Cells were stimulated with 5 μg/ml of concanavalin A for 24 h. |
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Applications |
Concanavalin A treatment could preferentially decrease the cell viability of CD8+ population prepared from the immunized mice. A certain group(s) of CD8+ population in the immunized mice might be highly susceptible to concanavalin A. The activation through T cell receptors, especially PKC activation, increases the sensitivity of CD8++ CTL to concanavalin A. |
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References: [1]. Kenichi T. et al. Concanamycin A, a vacuolar type H+-ATPase inhibitor, induces cell death in activated CD8+ CTL. Cytotechnology 1997 July; 25: 127–135. |
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| Cas No. | 11028-71-0 | SDF | |
| Formula | M.Wt | ||
| 溶解度 | Soluble in water | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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