Cy5-UTP, 10mM Sodium Solution |
カタログ番号GB20007 |
Cy5-UTPは、in vitro転写反応におけるRNAラベリングのためにUTPを置換できるシアニン5標識UTPアナログです。
Products are for research use only. Not for human use. We do not sell to patients.
Sample solution is provided at 25 µL, 10mM.
シアニン5-UTP(Cy5-UTP)は、T7 RNAポリメラーゼの基質としてUTPに代わり、in vitro転写を通じてオレンジ色に蛍光標識されたmRNAを生成することができます。励起/放出波長(nm):650/665nm [1]。 Cy5-UTPを基質として生成されたプローブは、特にCy5-UTPと組み合わせたデュアルカラー発現配列などのマルチカラー蛍光分析に適しています[2]。
References:
[1]. Custer TC, et al. In vitro labeling strategies for in cellulo fluorescence microscopy of single ribonucleoprotein machines. Protein Sci. 2017 Jul;26(7):1363-1379.
[2]. Guerra (2006) Analysis of oligonucleotide microarrays by 3' end labeling using fluorescent nucleotides and terminal transferase. Biotechniques 41 (1):53.
Cas No. | SDF | ||
Chemical Name | O=S(C1=CC2=C(N(CCCCCC(NC/C=C/C3=CN([C@H]4[C@H](O)[C@H](O)[C@@H](COP(OP(O)(OP(O)(O)=O)=O)(O)=O)O4)C(NC3=O)=O)=O)/C(C2(C)C)=C/C=C/C=C/C(C5(C)C)=[N+](CC)C6=C5C=C(S([O-])(=O)=O)C=C6)C=C1)(O)=O | ||
Formula | C45H58N5O22P3S2 | M.Wt | 1178.01 |
溶解度 | A solution in water | Storage | Store at -20°C,protect from light |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Prepare stock solution | |||
1 mg | 5 mg | 10 mg | |
1 mM | 0.8489 mL | 4.2444 mL | 8.4889 mL |
5 mM | 0.1698 mL | 0.8489 mL | 1.6978 mL |
10 mM | 0.0849 mL | 0.4244 mL | 0.8489 mL |
Average Rating: 5
(Based on Reviews and 30 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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