DC-Chol (hydrochloride) (Synonyms: Cholesteryl 3β-N-(dimethylaminoethyl)carbamate) |
| カタログ番号GC43385 |
DC-Chol (hydrochloride)はAβ40線維の形成を抑制することができ、DC-Chol (hydrochloride)は酸化hCTのアミロイド形成を抑制することができる。
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Cas No.: 166023-21-8
Sample solution is provided at 25 µL, 10mM.
DC-Chol (hydrochloride)はAβ40線維の形成を抑制することができ、DC-Chol (hydrochloride)は酸化hCTのアミロイド形成を抑制することができる。
DC-Chol (hydrochloride)については、Aβ40（30 µM）と低濃度のDC-Chol (hydrochloride)（50 ng/mL）を共存させた像ではAβ線維が観察された。しかし、500 µg/mL DC-Chol (hydrochloride)存在下では、画像中に繊維構造は認められなかった。DC-Chol/DOPEカチオン性リポソームは、強いエントロピー駆動型の表面静電相互作用によって、CT-DNAを適切に凝縮し、コンパクト化する[3]。仔牛胸腺DNA(CT-DNA)とカチオン性脂質3β-[N-(N',N'-dimethylaminoethane)-carbamoyl]コレステロール塩酸塩(DC-Chol(hydrochloride))とDOPEからなる混合カチオン性リポソームから構成されるリポプレックスを解析した。DC-Chol/DOPEリポソームは、平均流体力学的直径が（120±10）nm程度であり、強いエントロピー駆動型の静電相互作用によって、カチオン性表面でDNA断片をコンパクトに凝縮する。50mol%のDODACまたはDC-Chol (hydrochloride)と20mol%のDSPE-PEG(2000)で調製した製剤は、6.5～12.5時間の循環半減期を示した。DSPE-PEG(2000)を用いて調製したDC-Chol (hydrochloride)製剤は、PEGを含まない製剤と比較して、s.c.HT29腫瘍に3倍蓄積した。DC-Chol (hydrochloride)は、マクロサイトーシスとクラスリンを介したエンドサイトーシスにより内在化される。
References:
[1]. Elbassal EA, Liu H, et,al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. doi: 10.1021/acs.jpcb.5b09557. Epub 2015 Dec 23. PMID: 26652010; PMCID: PMC4959543.
[2]. Lantz R, Busbee B, et,al. Effects of disulfide bond and cholesterol derivatives on human calcitonin amyloid formation. Biopolymers. 2020 May;111(5):e23343. doi: 10.1002/bip.23343. Epub 2019 Dec 5. PMID: 31804717; PMCID: PMC9254112.
[3]. RodrÍguez-Pulido A, Ortega F, et,al. A physicochemical characterization of the interaction between DC-Chol/DOPE cationic liposomes and DNA. J Phys Chem B. 2008 Oct 2;112(39):12555-65. doi: 10.1021/jp804066t. Epub 2008 Aug 27. PMID: 18729499.
[4]. MuÑoz-Ubeda M, RodrÍguez-Pulido A, et,al. Effect of lipid composition on the structure and theoretical phase diagrams of DC-Chol/DOPE-DNA lipoplexes. Biomacromolecules. 2010 Dec 13;11(12):3332-40. doi: 10.1021/bm1008124. Epub 2010 Nov 8. PMID: 21058732.
[5]. Rapaka H, Manturthi S, et,al. Effect of Methylation of the Hydrophilic Domain of Tocopheryl Ammonium-Based Lipids on their Nucleic Acid Delivery Properties. ACS Omega. 2022 Apr 29;7(18):15396-15403. doi: 10.1021/acsomega.1c06889. PMID: 35571792; PMCID: PMC9096827.
[6]. Ho EA, Ramsay E, et,al. Characterization of cationic liposome formulations designed to exhibit extended plasma residence times and tumor vasculature targeting properties. J Pharm Sci. 2010 Jun;99(6):2839-53. doi: 10.1002/jps.22043. PMID: 20091826.
| 原子間力顕微鏡 (AFM) [1]: | |
基質 | Aβ40 |
準備方法 | Aβ40（30 μM）をpH 7.4のリン酸緩衝液に溶解し、Speci-Mixアリコットミキサーでインキュベートした。Aβ40を50 ng/mLのコレステロール-SO4、500 μg/mLのコレステロール-SO4、50 ng/mLのDC-Chol（塩酸塩）、および500 μg/mLのDC-cholesterolと共に処理した。 |
反応条件 | 50 ng/mLおよび500 μg/mLのDC-Chol（塩酸塩）をAβ40と共に17時間処理 |
アプリケーション | DC-Chol（塩酸塩）の場合、Aβ40（30 μM）と低濃度のDC-cholesterol（50 ng/mL）を共処理した画像でAβ線維が観察された。しかし、500 μg/mLのDC-cholesterolが存在する場合、線維構造は画像内に見られなかった。 |
参考文献: [1]. Elbassal EA, Liu H, et,al. Effects of Charged Cholesterol Derivatives on Aβ40 Amyloid Formation. J Phys Chem B. 2016 Jan 14;120(1):59-68. doi: 10.1021/acs.jpcb.5b09557. Epub 2015 Dec 23. PMID: 26652010; PMCID: PMC4959543. | |
| Cas No. | 166023-21-8 | SDF | |
| 同義語 | Cholesteryl 3β-N-(dimethylaminoethyl)carbamate | ||
| Chemical Name | (3β)-cholest-5-en-3-ol 3-[N-[2-(dimethylamino)ethyl]carbamate], monohydrochloride | ||
| Canonical SMILES | CC(C)CCC[C@@H](C)[C@@]1([H])CC[C@@]2([H])[C@]3([H])CC=C4C[C@@H](OC(NCCN(C)C)=O)CC[C@]4(C)[C@@]3([H])CC[C@@]21C.Cl | ||
| Formula | C32H56N2O2•HCl | M.Wt | 537.3 |
| 溶解度 | 10mg/mL in methanol, 5mg/mL in DMSO | Storage | Store at -20°C, protect from light |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.8612 mL | 9.3058 mL | 18.6116 mL |
| 5 mM | 0.3722 mL | 1.8612 mL | 3.7223 mL |
| 10 mM | 0.1861 mL | 0.9306 mL | 1.8612 mL |
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
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Quality Control & SDS
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- Purity: >97.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 38 reference(s) in Google Scholar.)
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