Erythrosin B (Erythrosin extra bluish) (Synonyms: Acid Red 51, Erythrosine, FD&C Red No. 3) |
| カタログ番号GC33466 |
エリスロシン B (エリスロシン エクストラ ブルー) は、食品および繊維産業で広く使用されている人工染料です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 16423-68-0
Sample solution is provided at 25 µL, 10mM.
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Erythrosin B is an artificial dye widely used in the food and textile industries. Erythrosin B is also a novel photosensitizer which has been used to develop animal models.
Only the two highest concentrations of Erythrosin B tested (50.0 and 70.0 μg/mL) are significantly different (p<0.05) from the vehicle control group when the Tail Moment and Tail Intensity, which represent the extent of DNA damage, are analyzed. Results show increased micronuclei (MNi) frequencies at six of the seven Erythrosin B concentrations (0.2 to 70.0 μg/mL) when compare to vehicle control group[1].
[1]. Chen W, et al. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8. [2]. Chequer FM, et al. Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem Toxicol. 2012 Oct;50(10):3447-51.
Cell experiment: | The alkaline single-cell gel electrophoresis assay (comet assay) is performed. Briefly, 2×105 HepG2 cells are seeded in a 24-well plate for 24 h. The cells are then treated with Erythrosine B at 0.1, 0.2, 2.0, 10.0, 25.0, 50.0 or 70.0 μg/mL (final concentration) for 4 h; vehicle control (0.7% DMSO) and positive control (doxorubicin0.3 μg/mL) treatments are also performed. The HepG2 cell suspension is mixed with 37°C low-melting point agarose and transferred to normal-melting point agarose-coated slides. One hundred randomly chosen nucleoids are analyzed per treatment, and a total of three independent experiments are performed[1]. |
References: [1]. Chen W, et al. Establishing an experimental rat model of photodynamically-induced retinal vein occlusion using erythrosin B. Int J Ophthalmol. 2014 Apr 18;7(2):232-8. | |
| Cas No. | 16423-68-0 | SDF | |
| 同義語 | Acid Red 51, Erythrosine, FD&C Red No. 3 | ||
| Canonical SMILES | O=C1OC2(C3=C(OC4=C2C=C(I)C([O-])=C4I)C(I)=C([O-])C(I)=C3)C5=C1C=CC=C5.[Na+].[Na+] | ||
| Formula | C20H6I4Na2O5 | M.Wt | 879.86 |
| 溶解度 | Water : 20.7 mg/mL (23.53 mM) | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.1365 mL | 5.6827 mL | 11.3654 mL |
| 5 mM | 0.2273 mL | 1.1365 mL | 2.2731 mL |
| 10 mM | 0.1137 mL | 0.5683 mL | 1.1365 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 8 reference(s) in Google Scholar.)
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