β-Estradiol - d3 |
| カタログ番号GC17258 |
β-エストラジオール - d3 (β-β-エストラジオール - d3) は、エストラジオールと標識された重水素です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 79037-37-9
Sample solution is provided at 25 µL, 10mM.
Estradiol, or 17β-estradiol, is a steroid and estrogen sex hormone, and the primary female sex hormone. It is named for and is important in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is essential for the development and maintenance of female reproductive tissues but it also has important effects in many other tissues including bone. β-Estradiol-d3 is the deuterated β-estradiol.
In vitro: Addition of β-estradiol in the cultures exerted a dose-dependent inhibition of IL-1-, TNF-, and IL-1 + TNF-induced production of bioassayable IL-6. β-estradiol also decreased the levels of the IL-6 mRNA. In addition, estradiol inhibited both TNF-induced IL-6 production and osteoclast development in primary bone cell cultures derived from neonatal murine calvaria [1].
In vivo: It was found that the density of synapses in the stratum radiatum of the hippocampal CA1 region in the adult female rat is sensitive to β-estradiol manipulation and fluctuates naturally as the levels of ovarian steroids vary during the 5 d estrous cycle. Synapse density then appears to cycle back to proestrus values over a period of several day [2].
Clinical trial: The combined regimen of β-estradiol and norethisterone acetate in postmenopausal Thai women was more effective in relieving the climacteric symptoms in women who used the drug than those who used the placebo. There was a high incidence of amennorhoea after 12 months. But there was also a high frequency of abnormal bleeding in the first 3 months of treatment. Counseling on the bleeding pattern and common side effects should be conducted before starting the treatment to increase the compliance [3].
Reference:
[1] Girasole G, Jilka RL, Passeri G, Boswell S, Boder G, Williams DC, Manolagas SC. 17 beta-estradiol inhibits interleukin-6 production by bone marrow-derived stromal cells and osteoblasts in vitro: a potential mechanism for the antiosteoporotic effect of estrogens. J Clin Invest. 1992 Mar;89(3):883-91.
[2] Woolley CS, McEwen BS. Estradiol mediates fluctuation in hippocampal synapse density during the estrous cycle in the adult rat. J Neurosci. 1992 Jul;12(7):2549-54.
[3] Limpaphayom KK, Bunyavejchevin S. Clinical effects of 17 beta-estradiol and norethisterone acetate in postmenopausal Thai women. J Med Assoc Thai. 2000 Apr;83(4):407-16.
| Cas No. | 79037-37-9 | SDF | |
| Chemical Name | (17β)-Estra-1,3,5(10)-triene-16,16,17-d3-3,17-diol | ||
| Canonical SMILES | O[C@@]1([2H])C([2H])([2H])C[C@]2([H])[C@]1(C)CC[C@@](C3=CC=C(O)C=C3CC4)([H])[C@]24[H] | ||
| Formula | C18H21D3O2 | M.Wt | 275.4 |
| 溶解度 | <20.65mg/ml in ethanol; <27.54mg/ml in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 3.6311 mL | 18.1554 mL | 36.3108 mL |
| 5 mM | 0.7262 mL | 3.6311 mL | 7.2622 mL |
| 10 mM | 0.3631 mL | 1.8155 mL | 3.6311 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >98.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 27 reference(s) in Google Scholar.)
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