Fetuin, Fetal Bovine Serum |
カタログ番号GC60166 |
フェチン、胎児牛血清は、しばしば多くの無血清培地に重要な補足物質である胎児牛血清からの糖タンパク質です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 9014-81-7
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Animal experiment [1]: | |
Animal models |
Male nude mice |
Preparation Method |
The prostate adenocarcinoma cell PC-3 (2 million cells) were injected on the upper half of the dorsal thorax of the mice. The tumors were allowed to grow for 6 weeks. The mice in the control group (no treatment) received 0.1 ml saline intraperitoneally for 5 days and the mice in the treated group received 50 mg/kg Fetuin, Fetal Bovine Serum. Treatment started the day after tumor inoculation for 5 days. Six weeks after the inoculation of tumor, mice were killed and tumors were removed and weighed. |
Dosage form |
Intraperitoneal injection, 50 mg/kg/d for 5d |
Applications |
Tumors (average weight 325 mg) developed in 13 of 13 mice in the control group (treated with saline), none of the nine mice treated with fetuin (50 mg/kg) developed any tumor. |
References: [1]: Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184. |
フェチン、胎児牛血清は、しばしば多くの無血清培地に重要な補足物質である胎児牛血清からの糖タンパク質です。牛フェチン準備はトリプシン活性を抑制し、多くの異なる培養系で細胞接着、成長、分化を促進しています[1]。フェチンはシスタチン超家族内のファミリーとして確立されています[2]。
フェチン、胎児性牛血清はHeLa細胞のS3株に対して、ガラス上での細胞接着と伸展(平坦化)を促進する活性があった[3]。フェチン、胎児性牛血清準備物は異なる種からの哺乳動物細胞の成長を刺激した。フェチン、胎児性牛血清(30-960 µg/ml)はHela細胞の成長を促進した[4]。フェチン、胎児性牛血清で培養されたLNCaP細胞核にはアポトーシスの特徴が見られた[5]。
フェチンは、胎児性牛血清を用いたP388白血病モデルにおいて、生存率を最大141%向上させました。また、PC-3前立腺がん動物モデルでは、フェチン(50mg/kg)がマウスにおける前立腺がんの形成を完全に抑制するようです[5]。
References:
[1]. Nie Z. Fetuin: its enigmatic property of growth promotion[J]. American Journal of Physiology-Cell Physiology, 1992, 263(3): C551-C562.
[2]. Brown W M, Dziegielewska K M, Saunders N R, et al. Fetuin坼an old friend revisited[J]. Bioessays, 1992, 14(11): 749-755.
[3]. Fisher H W, Puck T T, Sato G. Molecular growth requirements of single mammalian cells: The action of fetuin in promoting cell attachment to glass[J]. Proceedings of the National Academy of Sciences, 1958, 44(1): 4-10.
[4]. Marr A G M, Owen J A, Wilson G S. Studies on the growth-promoting glycoprotein fraction of foetal calf serum[J]. Biochimica et Biophysica Acta, 1962, 63(2): 276-285.
[5]. Yu C L, Tsai M H. Fetal fetuin selectively induces apoptosis in cancer cell lines and shows anti-cancer activity in tumor animal models[J]. Cancer letters, 2001, 166(2): 173-184.
Cas No. | 9014-81-7 | SDF | |
Formula | M.Wt | ||
溶解度 | Storage | Store at -20°C | |
General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
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(Based on Reviews and 13 reference(s) in Google Scholar.)GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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