Polybrene |
| カタログ番号GC19206 |
Polybreneは第四紀アモンニウム塩であり、ヘパリン中立剤として最初に臨床診療に導入される。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 28728-55-4
Sample solution is provided at 25 µL, 10mM.
Polybreneは第四紀アモンニウム塩であり、ヘパリン中立剤として最初に臨床診療に導入される。なお、低用量のPolybreneは体内と体外ではウイルス媒介遺伝子導入の効率的を促進するために一般的に使用される[2-5]。Polybreneは反対側の二重層間の静電反発を中和することにより、細胞表面上のウイルスの結合を促進できる[6-7]。
Polybrene（0-32 µg/ml; 24H）は濃度依存の効果を示している。4 µg/mlの低い濃度から始まると明確な神経炎のビーズが出現するが、8 µg/mLの濃度は神経炎の断片化を誘発する[8]。Polyethylene（8µg/mL）は間葉系幹細胞（MSC）の増殖速度を有意に低下させる[9]。Polybreneは脂肪分解の効率を著しく向上させ、さんざまな細胞株における血清媒介脂肪症の阻害を補償する[10]。
Polybrene（4 µg/µl iCV）は注射は3日目に運動機能阻害を示す鼠を引き起こす[8]。
References:
[1]. Weiss.W. A, Gilman, J. S, et,al. Heparin neutralization with polybrene administered intravenously. J. Am. Med. Assoc. 166, 603-607 (1958).
[2]. Davis, H. E., Morgan, J. R. et,al. Polybrene increases retrovirus gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes. Biophys. Chem. 97, 159-172 (2002).
[3]. Bellodi-Privato, M. et al. AAV gene transfer to the retina does not protect retrovirally transduced hepatocytes from the immune response. J. Mol. Med (Berl.). 82, 403-410 (2004).
[4]. Lauwers, E. et al. Neuropathology and neurodegeneration in rodent brain induced by lentiviral vector-mediated overexpression of alpha-synuclein. Brain Pathol. 13, 364-372 (2003).
[5]. Ferry, N., Duplessis, O., et,al. Retroviral-mediated gene transfer into hepatocytes in vivo. Proc. Natl Acad. Sci. USA 88, 8377-8381 (1991).
[6]. Sinn, P. L., Sauter, S. L. et,al. Gene therapy progress and prospects: development of improved lentiviral and retroviral vectors--design, biosafety, and production. Gene Ther. 12, 1089-1098 (2005).
[7]. Kafri, T. Lentivirus vectors: difficulties and hopes before clinical trials. Curr. Opin. Mol. Ther. 3, 316-326 (2001).
[8]. Bao F, Shi H, et,al. Polybrene induces neural degeneration by bidirectional Ca2+ influx-dependent mitochondrial and ER-mitochondrial dynamics. Cell Death Dis. 2018 Sep 20;9(10):966. doi: 10.1038/s41419-018-1009-8. PMID: 30237514; PMCID: PMC6148003.
[9].Lin P, Correa D, et,al. Polybrene inhibits human mesenchymal stem cell proliferation during lentiviral transduction. PLoS One. 2011;6(8):e23891. doi: 10.1371/journal.pone.0023891. Epub 2011 Aug 26. PMID: 21887340; PMCID: PMC3162604.
[10].Abe A, Miyanohara A, Friedmann T. Polybrene increases the efficiency of gene transfer by lipofection. Gene Ther. 1998 May;5(5):708-11. doi: 10.1038/sj.gt.3300652. PMID: 9797877.
| 細胞実験[1]: | |
細胞株 | 誘導性多能性幹細胞（IPSC）拡散ニューロシ系 |
準備方法 | |
反応条件 | 0-32 µg/ml; 24h |
アプリケーション | Polybreneは濃度依存の効果を示している。4 µg/mlの低い濃度から始まると明確な神経炎のビーズが出現するが、8 µg/mLの濃度は神経 |
| 動物実験 [2]: | |
動物モデル | 成年のBALB/C鼠（２－３か月） |
準備方法 | 4 µg/µlのPolybreneを37℃で15分間孵化し、脳室（ICV）で5 µLのの混合物を２０分間注射する。回転ロッドテストは3日で行う。 |
投与形態 | 4 µg/µl； iCV |
アプリケーション | Polybreneは注射は3日目に運動機能阻害を示す鼠を引き起こす |
References: | |
| Cas No. | 28728-55-4 | SDF | |
| Formula | (C13H30N2Br2)n | M.Wt | (374.2)n |
| 溶解度 | 10mg/ml in Water | Storage | Store at -20℃, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
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3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >95.00%
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- SDS (Safety Data Sheet)
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