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Uridine diphosphate glucose

カタログ番号GC37866

ウリジン二リン酸グルコース(ウラシル-二リン酸グルコース、UDPG)は、ヌクレオチド糖です。

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Uridine diphosphate glucose 化学構造

Cas No.: 133-89-1

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1mg
$167.00
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5mg
$352.00
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Sample solution is provided at 25 µL, 10mM.

Product Documents

Quality Control & SDS

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Protocol

Cell experiment [1]:

Cell lines

S. cerevisiae alg6 cells (PRY103) transformed with plasmids p426GPD or p426GPD-gpt1+

Preparation Method

The C-terminal end of S. pombe GT was mutated from PDEL to the S. cerevisiae retrieval signal HDEL, and the GT-encoding gene, gpt1+, was then inserted in high-copy number expression vectors (p426GPD) under the glyceraldehyde 3-P dehydrogenase promoter. S. cerevisiae alg6 cells (PRY103) were then transformed with the expression vectors with or without S. pombe gpt1+ gene.

Reaction Conditions

S. cerevisiae alg6 mutant (PRY103) cells transformed with p426GPD-gpt1+ or with p426GPD were incubated with [14C]glucose for 15 min in the presence or absence of DNJ. N-linked oliogosaccharides were regarded as the indication.

Applications

Uridine diphosphate glucose could be transported into the ER of Saccharomyces cerevisiae in vivo and into ER–containing microsomes in vitro. Uridine diphosphate glucose helps with the synthesis of b-1,6-glucan, one of Saccharomyces cerevisiae cell wall components, in the lumen of the endoplasmic reticulum (ER).

Animal experiment [2]:

Animal models

Balb/c mice; CRF1 mice; CD8 mice

Preparation Method

Mice of various strains, Balb/c, CD8, and CRF 1with and without tumor implants, maintained on Purina rat chow and tap water, were infused intraperitoneally with a phosphate-buffered saline (PBS) solution of Uridine diphosphate glucose at a constant rate of 0.8 ml/day for 5 days.

Dosage form

8 and 80 mM (0.28 and2.8 mmoles/kg body weight/day)

Applications

Uridine diphosphate glucose (UDPG) is shown to have tissue-specific effects that have proved to be of clinical value in the treatment of some liver ailments. The effects of Uridine diphosphate glucose on the levels of 5-phosphoribosyl pyrophosphate (PRPP) and PRPP synthetase in mouse liver, spleen and transplanted tumors are investigated to determine the mechanism of action with three stains of mice models.

References:

[1]. Olga C. et al. Uridine Diphosphate–Glucose Transport into the Endoplasmic Reticulum of Saccharomyces cerevisiae: In Vivo and In Vitro Evidence. Molecular Biology of the Cell 1999 Apr; 10, 1019–1030.

[2]. Yip LC, et al. Effects of uridine diphosphoglucose (UDPG) infusion on 5-phosphoribosyl pyrophosphate (PRPP) levels of mouse tissues. Biochem Pharmacol. 1987 Mar 1;36(5):633-7.

Background

ウリジン二リン酸グルコース(尿素二リン酸グルコース、UDPG)は、ヌクレオチド糖です。グルコシルトランスフェラーゼと呼ばれる酵素の基質であるブドウ糖の活性化形態として、ヌクレオチド糖代謝に使用されます。[1] ウリジン二リン酸グルコースは、臓器特異的な効果を示し、一部の肝臓障害の治療において臨床的価値が証明されています。また、肝臓で起こるグリコーゲン合成に関連する胆汁内ビリルビン代謝に多くの影響を与えることも知られています。[2]

体外研究により、UDPGの細胞代謝への影響は酵素誘導に限定されないことが示されています。他の研究では、酵素誘導を妨げる条件下でもUDPGが効果を持つ可能性があると報告しています。結果は、高極性化合物であるUDPGのかなりの量が変化せずに膜を通過することがわかりました。しかしながら、培養液に添加された大部分のUDPGは細胞内でペネトレーション後に切断されたグルコースリン酸として見つかりました。最終的に、細胞内に入ったすべてのUDPGはグルコースリン酸およびグルコースに分解されます。体外研究から得られたデータはG-6-Pが活性形態ではなく、ウリジンもUDP-G同様の変化を引き起こさないことを示しています。[2]

in vivo研究により、動物組織内のPRPPの細胞内レベルは、細胞外UDPGによって大きく影響を受けることが示されました。 UDPGによるPRPPレベルの変化は、線形的な用量関係ではありませんでした。 UDPGによって誘発されたPRPPの変化はまた、組織特異的でした:マウス肝臓は脾臓よりも感度が高かったです。この後者の特異性は、UDPGの治療上有益な効果と関連している可能性があります。[2]

References:
[1]. Rademacher T, Pet al. "Glycobiology". Annu Rev Biochem. 1988; 57: 785–838.
[2]. Yip LC, et al. Effects of uridine diphosphoglucose (UDPG) infusion on 5-phosphoribosyl pyrophosphate (PRPP) levels of mouse tissues. Biochem Pharmacol. 1987 Mar 1;36(5):633-7.

Chemical Properties

Cas No. 133-89-1 SDF
Canonical SMILES OC[C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)OP(OP(OC[C@@H]2O[C@@H](N3C=CC(NC3=O)=O)[C@H](O)[C@@H]2O)(O)=O)(O)=O)O)O)O
Formula C15H24N2O17P2 M.Wt 566.3
溶解度 Water : ≥ 50 mg/mL Storage Store at -20°C, protect from light, stored under nitrogen
General tips Please select the appropriate solvent to prepare the stock solution according to the solubility of the product in different solvents; once the solution is prepared, please store it in separate packages to avoid product failure caused by repeated freezing and thawing.Storage method and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored at -20°C, please use it within 1 month.
To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time.
Shipping Condition Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request.

Complete Stock Solution Preparation Table

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1 mg 5 mg 10 mg
1 mM 1.7658 mL 8.8292 mL 17.6585 mL
5 mM 0.3532 mL 1.7658 mL 3.5317 mL
10 mM 0.1766 mL 0.8829 mL 1.7658 mL
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