Cyanine5 alkyne |
| カタログ番号GC59102 |
Cyanine5 alkyne (Alkyne-Cy5) は、アジドタンパク質の標識に使用される蛍光色素であり、タンパク質の翻訳後修飾、グリコシル化などの分析に使用できます。Cyanine5 alkyne は、ミトコンドリア OXPHOS 阻害剤として使用して、癌幹の成長を阻害することもできます。セル (CSC)。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1223357-57-0
Sample solution is provided at 25 µL, 10mM.
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Cyanine5 alkyne for Click Chemistry, an analog of Cy5® alkyne. With this product, deeply colored, and photostable Cyanine5 fluorophore can be attached to various molecules via Click Chemistry reaction with azides.
This alkyne is non-water soluble, but it can be dissolved in DMF or DMSO prior to reaction, and added to aqueous reaction mixture. With our labeling protocol, the labeling reaction is very efficient, and high-yielding.
Various substrates bearing azides can be used for the labeling, including azido-labeled biomolecules, polymers, and solid surfaces.
| Cas No. | 1223357-57-0 | SDF | |
| Formula | C35H42ClN3O | M.Wt | 556.18 |
| 溶解度 | good in dichloromethane, DMF, DMSO, alcohols, very poorly soluble in water (200 mg/L = 0.4 mM) | Storage | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 1.798 mL | 8.9899 mL | 17.9798 mL |
| 5 mM | 0.3596 mL | 1.798 mL | 3.596 mL |
| 10 mM | 0.1798 mL | 0.899 mL | 1.798 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 19 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
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