Golimumab (Synonyms: CNTO-148) |
| カタログ番号GC66345 |
Golimumab (CNTO-148) は強力なヒト IgG1 TNFα です。アンタゴニストモノクローナル抗体。ゴリムマブには抗炎症活性があり、IL-6 と IL-1β を阻害します。製造。ゴリムマブは、TNF を標的にして中和することで作用し、軟骨と骨の炎症と破壊を防ぎます。ゴリムマブは抗がん作用があり、細胞アポトーシスを誘導します。ゴリムマブは、関節リウマチ、クローン病、がんの研究に使用できます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 476181-74-5
Sample solution is provided at 25 µL, 10mM.
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Golimumab (CNTO-148) is a potent human IgG1 TNFα antagonist monoclonal antibody. Golimumab has anti-inflammation activitity and inhibits IL-6 and IL-1β production. Golimumab acts via targeting and neutralizing TNF to prevent inflammation and destruction of cartilage and bone. Golimumab has the anticancer activity and induces cell apoptosis. Golimumab can be used for rheumatoid arthritis, Crohn's disease and cancer research[1][2][3].
Golimumab (CNTO-148) (0.1-10 μg /mL; 24-72 hours; transmembrane TNFα-transfected Jurkat cells) induces reductions in the viability of transmembrane TNFα-expressing cells[1].
Golimumab (CNTO-148) (10 μg /mL; 48 hours; transmembrane TNFα-transfected Jurkat cells) induces apoptosis, increase the levels of active caspase-3 and induces apoptotic DNA fragmentation[1].
Cell Viability Assay[1]
| Cell Line: | Transmembrane TNFα-transfected Jurkat cells |
| Concentration: | 0.1, 1 and 10 μg/mL |
| Incubation Time: | 24, 48 and 72 hours |
| Result: | Reduced cell viability in a dose- and time-dependent manner. |
Apoptosis Analysis[1]
| Cell Line: | Transmembrane TNFα-transfected Jurkat cells |
| Concentration: | 10 μg /mL |
| Incubation Time: | 48 hours |
| Result: | Had the percentage of apoptotic cells for 30.29%. |
Western Blot Analysis[1]
| Cell Line: | Transmembrane TNFα-transfected Jurkat cells |
| Concentration: | 10 μg /mL |
| Incubation Time: | 48 hours |
| Result: | Increased the levels of active caspase-3 and induces apoptotic DNA fragmentation. |
Golimumab (CNTO-148) (24 mg/kg; i.h.; daily, for 7 days; swiss-albino healthy male mice) inhibits oxidative stress, apoptotic cell death inflammatory response, thus improving renal function. Golimumab reduces all markers of kidney injury and attenuates cell death[2].
Golimumab (CNTO-148) (1-10 mg/kg; i.p.;daily; for 4 weeks; Tg197 transgenic mouse model) relieves TNFα-induced arthritis in a mouse model of human[3].
| Animal Model: | Swiss-albino healthy male mice[2] |
| Dosage: | 24 mg/kg |
| Administration: | Subcutaneous injection; daily, for 7 days |
| Result: | Reduced serum parameters like BUN, NGAL creatinine, cystatin C, and urinary parameters like KIM-1, NAG, albumin clusterin. |
| Animal Model: | Swiss-albino healthy male mice[2] |
| Dosage: | 24 mg/kg |
| Administration: | Subcutaneous injection; daily, for 7 days |
| Result: | Inhibited oxidative stress, reduces MDA concentrations and increases the GSH and catalase levels. |
| Animal Model: | Swiss-albino healthy male mice[2] |
| Dosage: | 24 mg/kg |
| Administration: | Subcutaneous injection; daily, for 7 days |
| Result: | Inhibitd cisplatin-induced inflammation, decreased TNF-α, including IL-6, IL-1β, MCP-1, ICAM-1, and TGF-β1 levels and increases IL-10 concentrations, reduced caspase 3 in cisplatin injected mice kidneys. |
| Animal Model: | Tg197 transgenic mouse model[3] |
| Dosage: | 1 and 10 mg/kg |
| Administration: | Intraperitoneal injection;for 4 weeks |
| Result: | Reduced the arthritic index. |
| Cas No. | 476181-74-5 | SDF | |
| 同義語 | CNTO-148 | ||
| Formula | M.Wt | ||
| 溶解度 | Storage | Store at -20°C | |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
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Quality Control & SDS
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- Purity: >99.00%
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Average Rating: 5 (Based on Reviews and 30 reference(s) in Google Scholar.)
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