HaloPROTAC 2 (Synonyms: HaloPROTAC 2) |
| カタログ番号GC32831 |
HaloPROTAC 2 (HaloPROTAC 2) は、E3 のリガンドと 21 原子長のリンカーのコンジュゲートです。リンカーのコネクターはハロゲン基です。 HaloPROTAC 2 には、VH032 ベースの VHL リガンドと 5 ユニットの PEG リンカーが組み込まれています。 HaloPROTAC 2 は、細胞ベースのアッセイで GFP-HaloTag7 の分解を誘導することができます。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 1799506-06-1
Sample solution is provided at 25 µL, 10mM.
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HaloPROTAC 2, a chloroalkane-containing PROTAC, induces degradation of HaloTag fusion proteins.
HaloPROTAC 1 and HaloPROTAC2, containing different linker lengths, are synthesized. Their ability to degrade GFP-HaloTag7, (stably expressed in HEK 293 cells) is tested tby flow cytometry, measuring changes in mean fluorescence intensity. While 24 hour treatment with HaloPROTAC 1 leads to less than 20% degradation, the longer HaloPROTAC2 leads to nearly 70% degradation of GFP-Halotag7 at 2.5 μM[1].
[1]. Buckley DL, et al. HaloPROTACS: Use of Small Molecule PROTACs to Induce Degradation of HaloTag Fusion Proteins. ACS Chem Biol. 2015 Aug 21;10(8):1831-7.
| Cas No. | 1799506-06-1 | SDF | |
| 同義語 | HaloPROTAC 2 | ||
| Canonical SMILES | O=C(NCC1=CC=C(C2=C(C)N=CS2)C=C1)[C@H]3N(C([C@H](C(C)(C)C)NC(COCCOCCOCCOCCOCCCCCCCl)=O)=O)C[C@H](O)C3 | ||
| Formula | C38H59ClN4O9S | M.Wt | 783.41 |
| 溶解度 | DMSO : ≥ 50 mg/mL (63.82 mM) | Storage | -80°C, protect from light, stored under nitrogen |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.2765 mL | 6.3824 mL | 12.7647 mL |
| 5 mM | 0.2553 mL | 1.2765 mL | 2.5529 mL |
| 10 mM | 0.1276 mL | 0.6382 mL | 1.2765 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
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- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 14 reference(s) in Google Scholar.)
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