Hypotaurine |
| カタログ番号GC14943 |
星状細胞におけるシステインからのタウリン生合成の中間体であるヒポタウリン (2-アミノエタンスルフィン酸) は、グリシン受容体の内因性阻害アミノ酸です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 300-84-5
Sample solution is provided at 25 µL, 10mM.
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Hypotaurine (2-aminoethanesulfinic acid), an intermediate in taurine biosynthesis from cysteine in astrocytes, is an endogenous inhibitory amino acid of the glycine receptor.
Hypotaurine and taurine are found to reside within the cytosolic compartment of the cell. The ratio of taurine to hypotaurine is approx 50:1. The cytosolic concentration of taurine is approx. 50 mM. The concentration of hypotaurine decreases by 80% when resting neutrophils are converted into actively respiring cells by exposure to opsonized zymosan[1]. Hypotaurine activates hypoxia signaling through the competitive inhibition of prolyl hydroxylase domain-2. This leads to the activation of hypoxia signaling as well as to the enhancement of glioma cell proliferation and invasion[2].
Hypotaurine has antinociceptive effects on thermal, mechanical, and chemical nociception in the spinal cord. In CCI rats, hypotaurine alleviates mechanical allodynia and thermal hyperalgesia. Intrathecal hypotaurine suppresses acute, inflammatory, and neuropathic pain. Hypotaurine may regulate nociceptive transmission physiologically by activating glycinergic neurons in the spinal cord[3].
References:
[1]. Green TR, et al. Antioxidant role and subcellular location of hypotaurine and taurine in human neutrophils. Biochim Biophys Acta. 1991 Jan 23;1073(1):91-7. https://www.ncbi.nlm.nih.gov/pubmed/1846756
[2]. Gao P, et al. Hypotaurine evokes a malignant phenotype in glioma through aberrant hypoxic signaling. Oncotarget. 2016 Mar 22;7(12):15200-14.
[3]. Hara K, et al. Antinociceptive effect of intrathecal administration of hypotaurine in rat models of inflammatory and neuropathic pain. Amino Acids. 2012 Jul;43(1):397-404. Hara K, et al. Antinociceptive effect of intrathecal administration of hypotaurine in rat models of inflammatory and neuropathic pain. Amino Acids. 2012 Jul;43(1):397-404.
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Animal experiment: |
Rats: A radiant heat source is focused on the middle part of the rat’s tail. The time interval from the onset of the stimulus until the tail flick response is measured using a tail flick unit. The intensity of the radiant heat is adjusted to give a tail flick latency of 4–5 s before the administration of hypotaurine or saline. In the absence of a response, the stimulus is terminated after 15 s (cutoff) to prevent tissue damage. The effects of hypotaurine (100, 200, 400, and 600 μg) on thermal nociception are assessed repeatedly for 120 min post-injection[3]. |
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References: [1]. Green TR, et al. Antioxidant role and subcellular location of hypotaurine and taurine in human neutrophils. Biochim Biophys Acta. 1991 Jan 23;1073(1):91-7. https://www.ncbi.nlm.nih.gov/pubmed/1846756 |
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| Cas No. | 300-84-5 | SDF | |
| Chemical Name | 2-aminoethanesulfinic acid | ||
| Canonical SMILES | OS(CCN)=O | ||
| Formula | C2H7NO2S | M.Wt | 109.14 |
| 溶解度 | PBS (pH 7.2): 10mg/mL | Storage | Desiccate at RT |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
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| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 9.1625 mL | 45.8127 mL | 91.6254 mL |
| 5 mM | 1.8325 mL | 9.1625 mL | 18.3251 mL |
| 10 mM | 0.9163 mL | 4.5813 mL | 9.1625 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
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Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 4 reference(s) in Google Scholar.)
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