KAT681 (T0681) (Synonyms: T0681) |
| カタログ番号GC31616 |
KAT681 (T0681) は肝臓選択的甲状腺刺激薬です。
Products are for research use only. Not for human use. We do not sell to patients.
Cas No.: 373641-87-3
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
KAT681 is a liver-selective thyromimetic.
The impact of the liver-selective thyromimetic KAT681 (T-0681) is investigated on lipoprotein metabolism. Prolonged treatment with KAT681 increases the hepatic expression of both low-density lipoprotein (LDL) receptor and scavenger receptor class B, type I without affecting cholesteryl ester transfer protein activity. Western blot showing human SR-BI (CLA-1) expression in normal HepG2 cells and in HepG2 cells loaded with AcLDL and subsequently incubated with vehicle or KAT681. SR-BI protein expression is markedly downregulated by incubation with 50 μg/mL AcLDL. This effect can not be reversed by addition of KAT681[1]
In preliminary dose-titration studies, a marked decrease of plasma cholesterol is observed at 36 nmoles/kg/day KAT681 (T-0681), whereas doses higher than 36 nmoles/kg/day show no further lipid-lowering effect. In the subsequent study, New Zealand White (NZW) rabbits are fed a 0.2% cholesterol diet and dosed with 36 nmoles/kg/day KAT681 or a respective placebo control for 4 weeks. KAT681 treatment results in a 60% decrease of plasma cholesterol and a 70% decrease of plasma triglycerides[1]. In preliminary dose-titration studies in wild-type (WT) mice, a marked increase of hepatic SR-BI expression at 36 nmol/kg/d KAT681 (T-0681), and a concomitant 50% decrease of plasma cholesterol are observed. Higher doses than 36 nmol/kg/d show no further lipid-lowering effect. KAT681 significantly increases hepatic LDLrs in SR-BI KO mice (2-fold of controls, P<0.01), along with a marked decrease in plasma cholesterol[2].
[1]. Tancevski I, et al. The thyromimetic T-0681 protects from atherosclerosis. J Lipid Res. 2009 May;50(5):938-44. [2]. Tancevski I, et al. The liver-selective thyromimetic T-0681 influences reverse cholesterol transport and atherosclerosis development in mice. PLoS One. 2010 Jan 15;5(1):e8722.
Cell experiment: | HepG2 cells are used. At a confluency of 70%, HepG2 cells are incubated with 50 μg/mL of acetylated LDL (AcLDL) for 24 h and subsequently treated with indicated amounts of KAT681 (0.5 μM) in serum-free medium for another 24 h. Cellular protein extraction and Western blot analysis are performed. |
Animal experiment: | Rabbits[1]Male NZW rabbits are subcutaneously implanted with Alzet osmotic pumps carrying KAT681 (36 nmoles/kg/day) in 1% DMSO/PBS or 1% DMSO/PBS alone as control for the entire duration of the studies. Rabbits are fed a 0.2% cholesterol and 3.5% fat diet or a 2% cholesterol and 5% fat diet. Food consumption is restricted to 100 g/day/animal. At the end of the studies, animals are fasted 5 h before the collection of blood samples, killed by a threefold overdose of pentobarbital, and organ biopsies snap-frozen.Mice[2]Male C57/B6 (WT) mice are fed a standard chow diet. After 2 weeks of acclimatization, mice are divided into two groups and subcutaneously implanted with Alzet micro-osmotic pumps carrying KAT681 in PBS (36 nmol/kg/d) or PBS alone as control for 14 days. After 14 days of treatment, animals are fasted for 5 h and anesthetized. Blood samples are taken, mice sacrificed by cervical dislocation, and organ biopsies are snap-frozen. Male SR-BI KO and LDLr KO mice are fed a standard chow diet, and subcutaneously implanted with Alzet micro-osmotic pumps carrying KAT681 in PBS (36 nmol/kg/d) or PBS alone as control. Mice are then fasted for 5 h, blood samples are taken, mice are sacrificed by cervical dislocation, and liver biopsies are snap-frozen. |
References: [1]. Tancevski I, et al. The thyromimetic T-0681 protects from atherosclerosis. J Lipid Res. 2009 May;50(5):938-44. | |
| Cas No. | 373641-87-3 | SDF | |
| 同義語 | T0681 | ||
| Canonical SMILES | O=C(O)CC(NC1=CC(C)=C(OC2=CC=C(O)C(C(C3=CC=C(F)C=C3)O)=C2)C(C)=C1)=O.[Na] | ||
| Formula | C24H22FNNaO6 | M.Wt | 462.42 |
| 溶解度 | Soluble in DMSO | Storage | Store at -20°C |
| General tips | Please select the appropriate solvent to prepare the stock solution according to the
solubility of the product in different solvents; once the solution is prepared, please store it in
separate packages to avoid product failure caused by repeated freezing and thawing.Storage method
and period of the stock solution: When stored at -80°C, please use it within 6 months; when stored
at -20°C, please use it within 1 month. To increase solubility, heat the tube to 37°C and then oscillate in an ultrasonic bath for some time. |
||
| Shipping Condition | Evaluation sample solution: shipped with blue ice. All other sizes available: with RT, or with Blue Ice upon request. | ||
| Prepare stock solution | |||
|
1 mg | 5 mg | 10 mg |
| 1 mM | 2.1625 mL | 10.8127 mL | 21.6254 mL |
| 5 mM | 0.4325 mL | 2.1625 mL | 4.3251 mL |
| 10 mM | 0.2163 mL | 1.0813 mL | 2.1625 mL |
Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
g
μL
Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)
Calculation results:
Working concentration: mg/ml;
Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )
Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.
Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.
Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
3. All of the above co-solvents are available for purchase on the GlpBio website.
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Average Rating: 5 (Based on Reviews and 24 reference(s) in Google Scholar.)
GLPBIO products are for RESEARCH USE ONLY. Please make sure your review or question is research based.
Required fields are marked with *